摘要
目的:研究白藜芦醇(RES)通过蛋白酪氨酸激酶2/信号转导子与激活子3(JAK2/STAT3)信号通路对人骨肉瘤体外细胞株MG-63细胞凋亡、侵袭和迁移的影响。方法:体外培养MG-63细胞,以不同浓度的RES作用于MG-63细胞。Annexin V-FITC/PI双染流式细胞术检测不同时间和不同浓度的RES对MG-63细胞凋亡的影响。划痕实验和Transwell实验检测不同时间和不同浓度的RES对MG-63细胞侵袭和迁移能力的影响。免疫印迹实验检测不同时间和不同浓度的RES对MG-63细胞磷酸化蛋白酪氨酸激酶2(p-JAK2)、磷酸化信号转导子与激活子3(p-STAT3)、凋亡相关蛋白B淋巴细胞瘤-2(Bcl-2)、Bcl-2家族促凋亡蛋白(Bax)及基质金属蛋白酶(MMP)-2、MMP-9表达的影响。结果:RES浓度越高,时间越久,MG-63细胞凋亡率越高(P<0.05)。RES浓度越高,MG-63细胞迁移和侵袭能力越弱(P<0.05)。RES处理MG-63细胞后其p-JAK2、p-STAT3、Bcl-2以及MMP-2、MMP-9的表达明显降低,而Bax蛋白表达明显升高,且p-JAK2、p-STAT3、Bax、Bcl-2以及MMP-2、MMP-9的表达水平变化具有RES浓度依赖性(P<0.05)。结论:RES可能通过调控JAK2/STAT3信号通路促使人骨肉瘤MG-63细胞凋亡,并抑制MG-63细胞侵袭和迁移。
Objective:To study the effect of resveratrol(RES)on the apoptosis,invasion and migration of human osteosarcoma cell line MG-63 through JAK2/STAT3 signaling pathway.Methods:MG-63 cells were cultured in vitro,and MG-63 cells were treated with different concentrations of RES.Annexin V-FITC/PI double staining was used to detect the effects of RES at different times and different concentrations on the apoptosis of MG-63 cells.Scratch test and Transwell invasion test were used to detect the effects of different time and different concentration of RES on the invasion and migration ability of MG-63 cells.Western blotting was used to detect the expression of phosphorylated protein tyrosine kinase 2(p-JAK2),phosphorylated protein tyrosine kinase 3(p-STAT3),apoptosis-associated protein B lymphocytoma-2(Bcl-2),Bcl-2 family of pro-apoptotic proteins(Bax),matrix metalloproteinase(MMP)-2 and MMP-9 in MG-63cells after treated with RES at different times points and concentrations.Results:The higher the RES concentration,and the longer the time,the higher the apoptosis rate of MG-63 cells(P<0.05).The higher the RES concentration,the weaker the migration and invasion ability of MG-63 cells(P<0.05).After RES treatment of MG-63 cells,the expression of p-JAK2,p-STAT3,Bcl-2,MMP-2 and MMP-9were significantly decreased,while the expression of Bax was significantly increased,and the expression levels of pp-JAK2,p-STAT3,Bax,Bcl-2,MMP-2 and MMP-9 were RES concentration-dependent(P<0.05).Conclusion:RES may promote the apoptosis of human osteosarcoma MG-63 cells by regulating the JAK2/STAT3 signaling pathway,and inhibit the invasion and migration ability of osteosarcoma cells.
作者
李家驹
刘振辉
孙天宇
姬昌彬
郭润涛
熊明月
LI Jia-ju;LIU Zhen-hui;SUN Tian-yu;JI Chang-bin;GUO Run-tao;XIONG Ming-yue(Department of Orthopaedics,New Area Hospital of the First Affiliated Hospital of Henan University of Science and Technology,L uoyang,Henan,471000,China;Department of Orthopaedics,Henan Provincial People's Hospital,Zhengzhou,Henan,450003,China)
出处
《现代生物医学进展》
CAS
2022年第8期1434-1438,共5页
Progress in Modern Biomedicine
基金
河南省科技厅基础与前沿技术研究计划项目(132300410469)。
