摘要
目的:探究岩藻多糖(FUC)作为功能食品对免疫低下小鼠免疫功能及肠道菌群的调节作用。方法:40只雄性BABL/C小鼠随机分为4组,用环磷酰胺构建免疫低下小鼠模型,每天灌胃给予干预组高、低剂量的岩藻多糖,对照组、模型组每天给予等体积生理盐水。通过酶联免疫吸附试验、流式细胞术、16S rRNA基因高通量测序等方法,检测免疫低下小鼠的免疫功能和肠道菌群。结果:免疫低下模型小鼠机体免疫功能明显下降,小鼠脾脏组织和肠道组织损伤严重;经FUC干预后,小鼠脾脏组织、回肠组织形态破坏得到明显改善,脏器指数、血清中免疫球蛋白和细胞因子的质量浓度明显提高(P<0.05)。菌群分析表明,属水平上,与正常组比,模型组小鼠盲肠内容物中拟杆菌含量显著升高(P<0.05),乳酸杆菌、罗氏菌含量显著降低(P<0.05);经FUC干预后,免疫低下小鼠肠道中失调的菌群得到良好的恢复;FUC干预的各组小鼠,肠道中菌群结构与正常组趋近。结论:FUC可有效提高免疫低下小鼠的免疫功能,并能有效缓解环磷酰胺造成的肠黏膜损伤,其作用机制可能与FUC对肠道菌群的调节作用有关。
Objective:To explore the regulation of fucoidan(FUC)as a functional food on immune function and intestinal flora in immunocompromised mice.Methods:Forty male BABL/C mice were randomly divided into 4 groups.The intervention group was given high and low doses of fucoidan for intragastric intervention every day for 28 consecutive days.The control group and model group were given equal volume of normal saline every day.Except for the normal group,the other groups were intraperitoneally injected with cyclophosphamide on the 24th day to construct an immunocompromised mouse model.Enzyme-linked immunosorbent assay,flow cytometry,HE staining,high-throughput sequencing of 16S rRNA gene of intestinal microbiota,etc.were used to detect the changes of mouse immune function and intestinal flora.Results:Compared with the normal group of mice,the immune function of the model group was significantly decreased,and the spleen and intestinal tissues were severely damaged.After FUC intervention,the morphological damage of the spleen and colon tissues was significantly improved(P<0.05).At the same time,FUC alleviated the increase of inflammatory factor levels in serum(P<0.05).Besides,the content of Bacteroides in the cecal content of the model group mice was significantly increased(P<0.05),Lactobacillus and Roseburia were significant decrease(P<0.05)compared with the normal group.After FUC intervention,the imbalanced intestinal flora was restored in model mice,and the composition structure of intestinal flora was approached the normal group.Conclusion:FUC can effectively improve the immune function and alleviate the intestinal mucosal damage in immunocompromised mice.The mechanism may be related to the change of intestinal flora.
作者
黄娟
黄金莉
孙佳悦
李瑶
李华军
Huang Juan;Huang Jinli;Sun Jiayue;Li Yao;Li Huajun(Department of Microecology,Dalian Medical University,Dalian 116044,Liaoning;Haibao Biological Technical Company,Dalian 116044,Liaoning)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2022年第5期92-102,共11页
Journal of Chinese Institute Of Food Science and Technology
关键词
岩藻多糖
免疫低下小鼠
免疫功能
肠道菌群
fucoidan
immunocompromised mice
immune function
intestinal flora
