摘要
目的:初步探讨益肾通络方对苯并(a)芘(BaP)染毒大鼠精子DNA碎片指数(DFI)的修复作用及可能机制。方法:将30只Wistar雄性大鼠随机分为3组,分别为空白组、BaP组、益肾通络方组,每组10只。除空白组外,其余组按照20 mg/(kg·d)的浓度进行BaP染毒,连续60 d。益肾通络方组自BaP染毒第31天起灌胃益肾通络方,共30 d。末次给药后取材,采用精子染色质结构分析法(SCSA)检测大鼠精子DFI;ELISA法检测血清FSH、LH、T水平、睾丸SOD、MDA、NO、ATP的水平。结果:与空白组相比,BaP组DFI[(12.46±3.07)%vs(4.23±1.40)%,P<0.05]明显升高,血清FSH[(2.53±0.28)U/L vs(1.76±0.31)U/L,P<0.05]、LH[(39.72±2.80)U/L vs(30.59±2.14)U/L,P<0.05]水平显著升高、T[(4.42±0.38)nmol/L vs(5.33±0.43)nmol/L,P<0.05]水平显著降低,睾丸SOD[(113.23±10.76)U/ml vs(166.18±3.74)U/ml,P<0.05]、ATP[(33.48±2.74)mol/L vs(41.23±2.21)mol/L,P<0.05]水平显著降低,MDA[(10.59±1.17)nmol/ml vs(7.55±0.93)nmol/L,P<0.05]、NO[(54.49±3.13)mol/L vs(44.23±4.47)mol/L,P<0.05]含量显著增高。与BaP组相比,益肾通络方组中以上各指标均明显恢复[DFI:(5.73±2.46)%,FSH:(2.07±0.45)U/L,LH:(33.94±4.44)U/L,T:(4.96±0.24)nmol/L,SOD:(135.22±7.26)U/ml,ATP:(38.26±2.14)mol/L,MDA:(8.37±1.29)nmol/ml,NO:(48.36±3.98)mol/L,P均<0.05]。结论:益肾通络方可以修复由BaP染毒引起的大鼠精子DNA损伤,其作用机制可能与抑制氧化损伤、调节生殖激素水平有关。
Objective:To investigate the repairing effect of Yishen Tongluo Prescription(YTP)on sperm DNA fragmentation index(DFI)in male rats exposed to benzo(a)pyrene(BaP)and its possible mechanism.Methods:Thirty Wistar male rats were equally randomized into a blank control,a BaP-exposure and a YTP intervention group,those in the latter two groups exposed to BaP at 20 mg/kg/d for 60 consecutive days,and those in the YTP intervention group treated intragastrically with YTP from the 31 st day of BaP exposure for a total of 30 days.After the last administration,the sperm DFI of the rats was detected by sperm chromatin structure analysis,the levels of FSH,LH and T in the serum and superoxide dismutase(SOD),malondialdehyde(MDA),nitric oxide(NO)and adenosine triphosphate(ATP)in the testis were measured by ELISA.Results:Compared with the blank controls,the rats in the BaP-exposure group showed significantly increased DFI([4.23±1.40]%vs[12.46±3.07]%,P<0.05),serum FSH([1.76±0.31]vs[2.53±0.28]U/L,P<0.05)and LH([30.59±2.14]vs[39.72±2.80]U/L,P<0.05),decreased levels of serum T([5.33±0.43]vs[4.42±0.38]nmol/L,P<0.05)and testicular SOD([166.18±3.74]vs[113.23±10.76]U/ml,P<0.05)and ATP([41.23±2.21]vs[33.48±2.74]mol/L,P<0.05),and elevated contents of MDA([7.55±0.93]vs[10.59±1.17]nmol/ml,P<0.05)and NO([44.23±4.47]vs[54.49±3.13]mol/L,P<0.05).All the above parameters returned to normal after YTP intervention(DFI:[5.73±2.46]%,FSH:[2.07±0.45]U/L,LH:[33.94±4.44]U/L,T:[4.96±0.24]nmol/L,SOD:[135.22±7.26]U/ml,ATP:[38.26±2.14]mol/L,MDA:[8.37±1.29]nmol/ml,NO:[48.36±3.98]mol/L),with statistically significant difference from those in the BaP-exposure group(all P<0.05).Conclusion:Yishen Tongluo Prescription can repair BaP-induced sperm DNA damage in male rats,which may be attributed to its effects of suppressing oxidative damage.
作者
赵士忠
张宸铭
王祖龙
王诗琦
李霄
陈如兵
华众
李凝华
姚均超
常贵峰
ZHAO Shi-zhong;ZHANG Chen-ming;WANG Zu-long;WANG Shi-qi;LI Xiao;CHEN Ru-bing;HUA Zhong;LI Ning-hua;YAO Jun-chao;CHANG Gui-feng(Henan University of Chinese Medicine,Zhengzhou,Henan 450046,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou,Henan 450003,China)
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2022年第4期326-331,共6页
National Journal of Andrology
基金
国家自然科学基金面上项目(82174377,81974573,81603632)
河南省科技攻关计划(192102310159)
河南省自然科学基金(222300420485)
河南省中医药科学研究专项课题(2022ZY1091)
国家中医临床研究基地科研专项(2021JDZX2056,2021JDZX2068)。
关键词
男性不育症
益肾通络方
苯并(A)芘
氧化应激
生殖激素
male infertility
Yishen Tongluo Prescription
benzo(a)pyrene
oxidative stress
sex hormones
