摘要
目的:分析足底电击应激对小鼠前额叶皮层、海马、杏仁核神经递质的影响。方法:选择健康雌性成年C57BL/6J小鼠,随机分为对照组和足底电击应激组。应激组给予不可逃避足底电击,隔日1次,持续14d。足底电击应激结束次日进行糖水偏爱实验评估小鼠抑郁样行为;提取小鼠前额叶皮层、海马、杏仁核,试剂盒检测谷氨酸和γ-氨基丁酸(GABA)含量,高效液相色谱方法检测去甲肾上腺素(NE)和5-羟色胺(5-HT)含量。结果:与对照组相比,足底电击应激小鼠糖水偏爱指数明显降低;足底电击应激小鼠杏仁核谷氨酸水平显著升高、前额叶皮层及海马GABA水平显著下降;前额叶皮层、海马谷氨酸及杏仁核GABA表达水平无显著差异;足底电击应激小鼠前额叶皮层5-HT、杏仁核NE水平显著升高;前额叶皮层、海马NE,以及海马、杏仁核5-HT表达水平无显著差异。结论:足底电击应激引起小鼠抑郁样行为,上调杏仁核谷氨酸、NE以及前额叶皮层5-HT的表达,下调前额叶皮层、海马GABA的表达。
Objective:To analyze the effects of foot-shock stress on neurotransmitters in the prefrontal cortex,hippocampus and amygdala of mice.Methods:Healthy female adult C57BL/6J mice were randomly divided into control group and foot-shock stress group.The rats in the stress group were given unavoidable foot-shock every other day for 14 days.The day after the end of the foot-shock stress,the sucrose preference test was performed to evaluate the depression-like behavior of the mice.Mouse prefrontal cortex,hippocampus,and amygdala were extracted,glutamate andγ-aminobutyric acid(GABA)contents were detected by commercial kits;Norepinephrine(NE)and serotonin(5-HT)contents were detected by high performance liquid chromatography(HPLC).Results:The sucrose preference index in the foot-shock stressed mice was significantly reduced compared with the control group;the amygdala glutamate level in foot-shock stress group was significantly increased,while the GABA level in the prefrontal cortex and hippocampus in foot-shock stress group were decreased significantly;the NE expression level in the amygdala and 5-HT expression level in the prefrontal cortex in foot-shock stress group were increased significantly.Conclusion:Foot-shock stress induced depression-like behavior in mice,and up-regulated prefrontal cortex 5-HT and amygdala glutamate and NE expressions;down-regulated prefrontal cortex and hippocampus GABA expressions.
作者
张益
黄雪妍
唐晓璐
薛进华
李延柠
Zhang Yi;Huang Xueyan;Tang Xiaolu;Xue Jinhua;Li Yanning(School of Basic Medicine,Gannan Medical University,Ganzhou 341000,China)
出处
《神经解剖学杂志》
CAS
CSCD
2022年第2期149-153,共5页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(81960341)
赣南医学院本科生科技创新项目(BKSZR201908)。
关键词
足底电击应激
前额叶皮层
海马
杏仁核
神经递质
小鼠
foot-shock stress
prefrontal cortex
hippocampus
amygdala
neurotransmitter
mouse
