摘要
以阿维菌素为起始原料,经催化加氢反应得到伊维菌素粗品。采用二维色谱纯化技术对伊维菌素粗品中的关键杂质进行分离提纯,鉴定了4个杂质,其中杂质1为阿维菌素B_(1a),杂质2为3′-OH-H_(2)B_(1a),杂质3为EP9.0中有关物质H,杂质4为EP9.0中有关物质K(H_(4)B_(1a)异构体)。
Using Avermectin as a starting material,we obtained crude Ivermectin by catalytic hydrogenation.Moreover,we separated and purified the key impurities in crude Ivermectin by two-dimensional chromatographic purification technology.Four impurities are identified,among which,impurity 1 is identified as Avermectin B_(1a),impurity 2 is identified as 3′-OH-H_(2)B_(1a),impurity 3 is identified as related substance H in EP9.0,and impurity 4 is identified as related substance K(H_(4)B_(1a) isomer)in EP9.0.
作者
高月麒
王克华
马志珺
林旸
李晓露
任风芝
GAO Yueqi;WANG Kehua;MA Zhijun;LIN Yang;LI Xiaolu;REN Fengzhi(NCPC New Drug Research & Development Co.,Ltd.,National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering & Technology Research Center,Shijiazhuang 052165,China;NCPC Aino Co.,Ltd.,Shijiazhuang 052165,China)
出处
《化学与生物工程》
CAS
2022年第5期26-31,共6页
Chemistry & Bioengineering
基金
河北省科技研发平台建设专项(199676154H)。
关键词
伊维菌素
合成
杂质
色谱纯化
结构鉴定
Ivermectin
synthesis
impurity
chromatographic purification
structure identification
