摘要
本试验旨在研究酶解鸡肉粉(EP)对初均重为(7.50±0.02)g的珍珠龙胆石斑鱼(E.fuscoguttatus♀×E.lanceolatus♂)肌肉营养品质及肌肉生长相关因子表达的影响。分别配制以0(EP0组,作为对照组)、3%(EP3组)、6%(EP6组)、9%(EP9组)、12%(EP12组)、15%(EP15组)和18%(EP18组)鸡肉粉等量替代鱼粉的7种等氮等脂的试验饲料。630条珍珠龙胆石斑鱼被随机分为7组,每组3个重复,每个重复30尾鱼,放养于300 L玻璃钢桶。试验鱼每天表观饱食投喂2次(08:00和16:00),试验期共计56 d。结果表明:酶解鸡肉粉替代不同比例鱼粉对珍珠龙胆石斑鱼背肌的水分、粗蛋白质、粗脂肪和粗灰分含量未产生显著影响(P>0.05)。EP6组肌肉蒸煮损失显著低于EP0、EP3、EP9和EP18组(P<0.05),硬度、胶黏性和咀嚼性显著高于EP12和EP15组(P<0.05),生肌调节因子家族中肌细胞因子5(Myf5)和肌球蛋白重链(MyHC)的相对表达量显著高于EP12、EP15和EP18组(P<0.05),肌肉生长正调控因子卵泡抑素(FST)的相对表达量显著高于EP0组(P<0.05),肌肉生长负调控因子肌肉生长抑制素(MSTN)的相对表达量显著低于EP0和EP3组(P<0.05)。综上所述,6%酶解鸡肉粉等量替代鱼粉能够改善珍珠龙胆石斑鱼背肌的系水力和质构特性,提高生肌调节因子家族及肌肉生长正调控因子的表达,抑制肌肉生长负调控因子的表达。
The purpose of this experiment was to investigate the effects of replacing fish meal with enzyme-digested poultry by-product meal(EP) on muscle quality and expression of muscle growth-related factors of hybrid grouper(Epinephelus fuscoguttatus ♀×E.lanceolatus ♂) with an initial body weight of(7.50±0.02) g.Seven iso-nitrogenous and iso-lipidic experimental diets were formulated and fed to hybrid grouper with 0(EPO group,as control group),3%(EP3 group),6%(EP6 group),9%(EP9 group),12%(EP12 group),15%(EP15 group),and 18%(EP18 group) of EP as an equivalent replacement for fish meal.A total of 630 hybrid groupers were randomly divided into 7 groups of 3 replicates each,with 30 fish per replicate stocked in 300 L fiberglass drums.Apparent satiation was fed twice daily(08:00 and 16:00) for a total of 56 days.The results showed that the moisture,crude protein,ether extract and ash contents in muscle of groupers were not significantly affected by the replacement ratio of fish meal by EP(P>0.05).Muscle cooking loss in the EP6 group was significantly lower than that in the EP0,EP3,EP9 and EP18 groups(P<0.05).Muscle hardness,gumminess and chewiness in the EP6 group were significantly higher than those in the EP12 and EP15 groups(P<0.05).Muscle myogenic regulatory factor’s family myogenic factor 5(Myf5) and myosin heavy chain(MyHC) relative expression levels in the EP6 group were significantly higher than those in the EP12,EP15 and EP18 groups(P<0.05).The relative expression level of follistatin(FST),a positive regulator of muscle growth,was significantly higher in the EP6 group than that in the EP0 group(P<0.05).The relative expression level of myostatin(MSTN),a negative regulator of muscle growth,was significantly lower in the EP6 group than that in the EP0 and EP3 groups(P<0.05).In conclusion,6% EP as an equivalent replacement for fish meal can significantly improve the water holding capacity and textural properties of muscle,and significantly increase the expression of myogenic regulatory factors and positive regul
作者
杨烜懿
宋紫菱
植心妍
赵旭民
王光辉
迟淑艳
谭北平
YANG Xuanyi;SONG Ziling;ZHI Xinyan;ZHAO Xumin;WANG Guanghui;CHI Shuyan;TAN Beiping(Laboratory of Aquatic Animal Nutrition and Feed,Guangdong Ocean University,Zhanjiang 524088,China;Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province,Zhanjiang 524088,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang),Zhanjiang 524025,China;Yichang Huatai Biological Technology Co.,Ltd.,Yichang 443500,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2021年第12期6999-7011,共13页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家重点研发计划(2019YFD0900200)
南方海洋科学与工程广东省实验室(湛江)(ZJW⁃2019⁃06)
广东省现代农业产业技术体系创新团队建设项目(2020KJ150)
广东省普通高校重点领域专项(2020ZDZX1034)。
关键词
酶解鸡肉粉
珍珠龙胆石斑鱼
肌肉品质
肌肉生长相关因子
enzyme-digested poultry by-product meal
hybrid grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂)
muscle quality
muscle growth related factors
