摘要
采用超声辅助酶法制备南极磷虾抗菌肽,以抑菌圈直径为指标筛选蛋白酶,得到中性蛋白酶为最佳试验用酶。后续采用超声辅助中性蛋白酶酶解法制备南极磷虾抗菌肽,以抗菌肽得率及抑菌圈直径为指标,考察底物浓度、超声时间、超声功率、酶解温度、酶解时间和加酶量六个因素对抗菌肽得率的影响。从中选取影响抗菌肽得率的三个主要因素:底物浓度、酶时间、超声功率,以抗菌肽得率为响应值,采用响应面法进行工艺优化。结果表明:三个因素对得率的影响大小为底物浓度>超声功率>酶解时间,得到最佳制备条件为:底物浓度21%、酶解温度50℃、超声功率120W、超声时间25min、酶解时间4h、加酶量6000U/g,此条件下抗菌肽得率为51.24%,抑菌圈直径为20.8mm,均显著高于常规酶解法(P<0.05),表明此优化工艺可行。为南极磷虾抗菌肽的潜在开发应用提供了思路和方法。
The antibacterial peptide of Antarctic krill was prepared by ultrasonic-assisted enzyme method.The protease was screened using antibacterial circle diameter as the index,and neutral protease showed to be the best enzyme.In the follow-up experiment,the antibacterial peptide of Antarctic krill was prepared by ultrasonicassisted neutral protease enzymolysis.Taking the yield of antibacterial peptide and the diameter of antibacterial circle as indexes,the effects of substrate concentration,ultrasonic time,ultrasonic power,enzymolysis temperature,enzymolysis time and enzyme amount on antibacterial peptide yield were investigated.The substrate concentration,enzymolysis time and ultrasonic power were selected as the factors with the greatest influence on the yield of antimicrobial peptides.The yield of antimicrobial peptides was taken as the response value,and the process was optimized by response surface method.The results showed that the influence of three factors on the yield was substrate concentration>ultrasonic power>enzymolysis time.The optimum preparation conditions were as follows:substrate concentration 21%,enzymolysis temperature 50℃,ultrasonic power 120W,ultrasound time 25min,enzymolysis time 4 h and enzyme amount 6000U/g.Under these conditions,the yield of antimicrobial peptides was 51.24%.The diameter of antibacterial circle was 20.8mm,which was significantly higher than that of traditional enzymolysis(P<0.05).It shows that the optimized process is feasible.It provides ideas and methods for the potential application development of antimicrobial peptides from Antarctic krill.
作者
许依能
纪登杰
杨威
马洁
陈丽
XU Yineng;JI Dengjie;YANG Wei;MA Jie;CHEN Li(College of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222005;Jiangsu Marine Biological Industry Technology Collaborative Innovation Center,Lianyungang 222005;Jiangsu Institute of Marine Resources Development,Jiangsu Ocean University,Lianyungang 222005)
出处
《中国食品添加剂》
CAS
北大核心
2022年第5期73-80,共8页
China Food Additives
基金
江苏省海洋科技创新专项项目(HY2018-10)
江苏省“333工程”高层次人才培养资金资助项目(BRA2014111)
连云港市“521高层次人才培养工程”资助项目(LYG52105-2018028)。
关键词
南极磷虾
超声波
抗菌肽
响应面
得率
Antarctic krill
ultrasonic
antimicrobial peptides
response surface
yield
