摘要
以多年生黑麦草(Lolium perenne L.)品种麦迪(Mathilde)和绅士(Esquire)为供试材料,采用0、25、50、100和200mmol/L NaCl共5个处理,研究不同浓度NaCl处理对多年生黑麦草生理特性以及耐盐基因表达模式的影响。结果显示:两个黑麦草品种地上生物量、根重、株高、PSⅡ最大光化学量子产量(Fv/Fm)在不同浓度NaCl处理下均受到不同程度抑制;两个品种黑麦草地上部和根部K^(+)含量在盐胁迫下均持续下降,Na^(+)含量显著升高,但与绅士相比,麦迪能够在轻度盐胁迫(25和50mmol/L)下维持其地上部K^(+)含量的相对稳定和较高的K^(+)/Na^(+)比率;两个品种黑麦草可溶性糖含量均随着NaCl处理浓度的升高呈现出上升趋势;耐盐候选基因LpNHX1、LpSKOR、LpHKT1;5的表达量在两个品种黑麦草中均能受到外界盐胁迫显著诱导而上调。
This study explored the effects of different concentrations(0,25,50,100,200 mmol/L)of NaCl on physiological characteristics and salt-tolerant genes expression patterns of perennial ryegrass(Lolium perenne L.)varieties“Mathilde”and“Esquire”.The results showed that the shoot biomass,root weight,plant height,and maximum photochemical quantum yield of PSⅡ(Fv/Fm)of the two ryegrass cultivars were inhibited under different concentrations of NaCl treatments.The K^(+)concentration in the shoot and root of both ryegrass cultivars decreased under different concentrations of NaCl treatments,while Na^(+)concentration increased significantly.However,compared with“Esquire”,“Mathilde”maintained a relatively stable K^(+)concentration and a higher K^(+)/Na^(+)ratio in shoot under mild salt stress(25 and 50 mmol/L NaCl).The soluble sugar content of the two cultivars increased with the elevation of NaCl concentration.The gene expression levels of LpNHX1,LpSKOR,and LpHKT1;5 were significantly up-regulated under salt stress in both cultivars.
作者
宋鑫
王加杰
徐杉
张美艳
何映辉
SONG Xin;WANG Jia-jie;XU Shan;ZHANG Mei-yan;HE Ying-hui(Key Laboratory of Regulation of Crop Growth and Development in Jiangxi Province/College of Life Science and Resources and Environment,Yichun University,Yichun 336000,China;College of Life Science,Chongqing Normal University,Chongqing 401331,China;Academe of Grassland and Animal Science,Kunming 650000,China;College of Animal Science and Veterinary Medicine,Henan Agriculture Universital,Zhengzhou 450000,China)
出处
《中国草地学报》
CSCD
北大核心
2022年第5期58-65,共8页
Chinese Journal of Grassland
基金
江西省作物生长发育调控重点实验室开放基金课题基金项目(JXJJ202104)
宜春学院博士科研启动项目(2103360119048)。
