摘要
以大田环境内多年生荒漠植物白刺(Nitraria tangutorum)为研究对象,采用扫描电子显微镜对不同外源脯氨酸质量浓度处理下白刺叶片气孔进行微观结构观察并测定叶片表皮气孔器长度、宽度、面积和密度,比较同一指标在不同脯氨酸质量浓度处理下的差异。采用室外试验研究自然干旱胁迫下喷施质量浓度为50、100、150、200、250 mg·L^(-1)的外源脯氨酸(Proline,缩写为Pro或P)研究白刺的耐旱性。观察结果显示,白刺叶片气孔保卫细胞为肾形,气孔在叶片表皮随机分布,气孔器多为无规则型,气孔呈椭圆形且叶片表面蜡质较少,并且叶片细胞出现褶皱和下陷。与对照相比,在不同质量浓度处理和不同采样时间时,气孔长度、宽度及面积下降,气孔密度增大,随着浓度的升高以及采样时间的变化,气孔长度整体呈现下降趋势,当浓度达到200 mg·L^(-1)时,下降幅度增大,宽度和面积整体呈现先降低后升高再降低的趋势,而气孔密度则整体呈现上升趋势,其中,气孔长度、宽度和面积在质量浓度为50 mg·L^(-1)、采样第1天时达到最大值,气孔密度在质量浓度为150 mg·L^(-1)、采样第9天时达到最大值。此外,气孔长度在不同浓度脯氨酸处理下第1、6和9天存在显著差异,而气孔宽度和面积差异不显著,除第0天外,气孔密度因脯氨酸质量浓度不同均差异显著(P<0.05)。研究结果表明,自然干旱胁迫下对白刺叶片喷施不同质量浓度的外源脯氨酸,可减小气孔长度、宽度和面积,而增加气孔密度,以期为白刺抗旱提供理论依据。
Taking Nitraria tangutorum,a perennial desert plant in the field environment,as material,scanning electron microscope was used to observe the microstructure of N.tangutorum leaf stomata underdifferent exogenous proline concentrations,and the length,width,area and density of the leaf epidermalstomatal apparatus were measured respectively,and the difference of the same index under different prolineconcentration treatments were compared.The concentrations of exogenous proline spraying were set at 50,100,150,200,250 mg·L^(-1)respectively(Proline,abbreviated as Pro or P)under natural drought stress,and thedrought tolerance of N.tangutorum were tested respectively.The observation results showed that the stomataguard cells of N.tangutorum leaves were kidney-shaped,and the stomata were randomly distributed in the leafepidermis.The stomatal apparatus was mostly irregular,and the stomata were oval and leaf surface waxy less.And the leaf cells appeared folds and sinks.Compared with the control,under different concentration treatments and different sampling days,the stomata length,width and area decreased,and the stomata density increased.As the concentration increased and the number of sampling days changed,the overall stomata length showed adownward trend.When the concentration reached at 200 mg·L^(-1),the decreasing amplitude increased,and theoverall width and area showed a trend of first decreased,then increased and then decreased,while the stomatadensity showed an overall upward trend.Among them,the stomata length,width and area were at aconcentration of 50 mg·L^(-1),the number of sampling days reached the maximum on the first day,and thestomatal density reached the maximum at the concentration of 150 mg·L^(-1)and the ninth day of the sampling days.In addition,the stomata length differed significantly on the first day,the sixth day,and the ninth day under thetreatment of different concentrations of proline,while the stomata width and area were not significantly different.Except for the beginning,the stomata density
作者
魏斌
李毅
苏世平
WEI Bin;LI Yi;SU Shiping(College of Forestry,Gansu Agricultural University,Lanzhou 730070)
出处
《植物研究》
CAS
CSCD
北大核心
2022年第3期492-501,共10页
Bulletin of Botanical Research
基金
甘肃农业大学林学一级学科建设基金项目(GAU-XKJS-2018-103,GAU-XKJS-2018-102)
国家自然科学基金项目(32060335)。
关键词
干旱胁迫
白刺
外源脯氨酸
叶表面
气孔微观结构
drought stress
Nitraria tangutorum
exogenous proline
leaf surface
stomata microstructure
