摘要
目的探索肠道细菌菌群失调与原发性肾小球疾病患者炎症反应及体液免疫的相关性,为该病的早期诊断及治疗提供理论依据。方法选取2017年1月-2020年10月本院收治的原发性肾小球疾病患者85例(研究组),85例同期门诊健康体检者85例(对照组)。收集两组受试者一般临床资料进行比较;采集受试者血液,采用免疫荧光试剂盒检测血清超敏C反应蛋白(hs-CRP)、白细胞介素-6(IL-6)、白细胞介素-18(IL-18)和肿瘤坏死因子-α(TNF-α);采用全自动生化分析仪检测血清C3、C4、IgG、IgA、IgM水平;采集患者粪便标本进行培养,分析肠道菌群结构与炎症因子和体液蛋白的相关性。结果原发性肾小球疾病患者粪便大肠埃希菌丰度为(10.42±0.34)logN/g,酵母菌丰度为(3.56±0.74)logN/g,双歧杆菌丰度为(7.08±0.85)logN/g;健康对照组分别为(9.68±0.82)logN/g、(3.31±0.54)logN/g和(8.99±1.71)logN/g,差异均有统计学意义(均P<0.01)。原发性肾小球疾病患者粪便双歧杆菌和大肠埃希菌丰度与血清TNF-α、IL-6、IL-18和hs-CRP等炎症因子水平均存在相关性(均P<0.05);乳酸杆菌、双歧杆菌和大肠埃希菌丰度与血清IgA、IgG、IgM、C3和C4水平亦存在相关性(均P<0.05)。结论原发性肾小球疾病患者肠道菌群结构发生改变,炎症因子和免疫蛋白水平异常,且肠道菌群分布与炎症因子和免疫蛋白水平存在显著相关性,临床上可根据患者肠道菌群分布情况进行诊断并采取针对性治疗。
Objective To explore the correlation between intestinal bacterial flora imbalance and inflammatory response and humoral immunity in patients with primary glomerular disease,in order to provide a theoretical basis for early clinical diagnosis and treatment of primary glomerular disease.Methods From January 2017to October 2020,85patients with primary glomerular disease and 85outpatients with physical examination in the same period were selected as the research objects.They were divided into the research group and the control group.The general clinical data of the two groups,including age,gender,blood lipid,blood glucose and blood pressure,were collected and compared.Collect 5ml of fasting venous blood in the morning,centrifuge the upper serum,and detect serum high-sensitivity C-reactive protein(hs CRP),interleukin-6(IL-6),interleukin-18(IL-18)and tumor necrosis factor by enzyme-linked immunosorbent assay-α(TNF-α).The levels of serum C3,C4,IgG,IgA and IgM were detected by automatic biochemical analyzer;5g fecal samples were collected for culture,and the correlation between intestinal flora structure and inflammatory factors and humoral proteins was analyzed.Results The Escherichia coli(10.420.34)logN/g and Yeast(3.560.74)logN/g in the study group were significantly higher than those in the healthy population(9.680.82)logN/g and(3.310.54)logN/g.Bifidobacterium(7.080.85)logN/g was significantly lower than that of healthy population(8.99±1.71)(all P<0.05).Bifidobacteria and E.coli and TNF in patients with primary glomerulus-α(r=0.417,0.765),IL-6(r=0.358,0.674),IL-18(r=0.539,0.718)and hs CRP(r=0.617,0.693)were correlated with inflammatory factors(all P<0.05).Lactobacillus,Bifidobacteri-umand E.coli were also correlated with IgA(r=0.412,0.426,0.782),IgG(r=0.398,0.375,0.793),IgM(r=0.466,0.369,0.745),C3(r=0.437,0.365,0.719)and C4(r=0.551,0.348,0.774)(all P<0.05).Conclusion Changes in intestinal flora structure,abnormal levels of inflammatory factors and immune proteins,and significant correlation between intestinal flo
作者
吴晶
张君林
汤琼
熊华
孟淼
WU Jing;ZHANG Jun-lin;TANG Qiong;XIONG Hua;MENG Miao(Department of Nephrology,Affiliated Hospital of Jiangnan University,Wuxi,Jiangsu 214000,China;Department of Gastroenterology,Wuxi Second People's Hospital)
出处
《中国病原生物学杂志》
CSCD
北大核心
2022年第2期220-223,228,共5页
Journal of Pathogen Biology
关键词
肠道菌群
原发性肾小球
炎症因子
血清补体
intestinal flora
primary glomerular disease
inflammation index
serum complement
