摘要
Bacteria-associated infection and poor osseointegration are two main reasons for orthopedic implant failure.Ti-Cu alloy exhibited excellent antibacterial property,but still presented unsatisfied osteogenic activities.Therefore,Ti-Cu alloy was surface modified by an alkali-heat treatment in this paper to improve the osteogenic ability without reduction in antibacterial ability.A TiO_(2)/CuO/Cu_(2)O composite coating with nanostructure was deposited on Ti-Cu alloy.The coating showed increased roughness and great hydrophilicity.Antibacterial tests indicated that the modified Ti-Cu alloy exhibited stronger antibacterial ability against Staphylococcus aureus(S.aureus)than Ti-Cu alloy.Meanwhile,cell experiments demonstrated that the composite coating promoted initial adhesion and spreading of MC3T3-E1 cells,enhanced alkaline phosphatase(ALP)activities as well as extracellular matrix(ECM)mineralization,and significantly upregulated osteogenesis-related gene expressions.It was suggested that the nano-structured TiO_(2)/CuO/Cu_(2)O coating on Ti-Cu alloy might provide a potential strategy for orthopedic implant failure.
基金
financial support from the National Natural Science Foundation of China(No.31971253)。
