摘要
极长链脂肪酸延伸酶蛋白家族(elongation of very-long-chain fatty acids,ELOVLs)是一类催化脂肪酸合成的限速酶,主要调控血脂、血糖及一些代谢疾病的发生。为探究绵羊ELOVL5基因的结构和功能,本研究对该基因及其编码产物进行了生物信息学分析。结果显示,绵羊ELOVL5基因编码737个氨基酸,其编码蛋白分子式为C_(1656)H_(2448)N_(416)O_(415)S_(16),其分子质量为35335.39 Daltions,理论等电点为9.47,估计半衰期为30 h,不稳定性指数为33.35。亚细胞定位主要位于内质网中(55.6%),ELOVL5基因编码蛋白没有信号肽序列,不属于分泌蛋白。该蛋白存在多个跨膜区域,属于跨膜蛋白,存在7个保守结构域,并且为亲水性蛋白,二级结构主要以α螺旋和无规则卷曲为主,三级结构主要由α螺旋缠绕折叠而成。
Very long chain fatty acids extend enzyme protein family(elongation of very long-chain fatty acids,ELOVLs)is a kind of fatty acid catalytic synthesis of speed limit of enzymes.It mainly regulates the occurrence of blood lipid,blood glucose and some metabolic diseases.To explore the structure and function of ELOVL5 gene in sheep.this gene and its encoding protein were analyzed by bioinformatics.The results showed that the sheep ELOVL5 gene encoded 737 amino acids,and the formula was C_(1656)H_(2448)N_(416)O_(415)S_(16).The molecular weight of the protein was 35335.39 Daltions,the theoretical isoelectric point was 9.47,the estimated half-life was 30h,and the instability index was 33.35.The subcellular localization was mainly located in the endoplasmic reticulum(55.6%).The protein encoded by ELOVL5 gene had no signal peptide sequence and was not secretory protein.This protein was transmembrane protein with multiple transmembrane regions.had 7 conserved domains,and was hydrophilic.The secondary structure mainly consist of alpha helix and random coil,the tertiary structure is mainly composed of winding and folding ofαhelix.
作者
张小雪
赵利明
刘佳
杨晓斌
李冲
ZHANG Xiaoxue;ZHAO Liming;LIU Jia;YANG Xiaobin;LI Chong(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou Gansu 730070,China)
出处
《甘肃农业科技》
2022年第4期24-29,共6页
Gansu Agricultural Science and Technology
基金
国家畜禽良种联合攻关计划项目(19210365)
甘肃农业大学伏羲青年英才培养计划项目(Gaufx-03Y11)。
