摘要
以西伯利亚鲟鱼肝脏为原料,分离纯化出鲟鱼肝脏铁蛋白(liver ferritin of Acipenser baerii,ABLF)。采用电泳、紫外、动态光散射(dynamic light scattering,DLS)、傅里叶变换红外光谱等分析5个不同热处理温度(60~100℃)对ABLF稳定性的影响,同时探究25~65℃热处理温度对ABLF铁释放速率快慢的影响及在4、25℃条件下ABLF的贮藏稳定性。研究表明:随着热处理温度的升高(60~100℃),ABLF的溶解度和铁含量逐渐降低,蛋白聚集体含量增加;红外光谱分析显示ABLF中α-螺旋相对含量逐渐降低,无规卷曲相对含量整体呈增加趋势,以上现象在80~100℃范围内尤为明显,而在60~80℃范围内变化较小,表明ABLF在60~80℃具有良好的热稳定性。ABLF的铁释放速率随温度升高(25~65℃)而增加。4℃条件下ABLF贮藏稳定性高于25℃。本研究将为ABLF的加工及利用提供理论依据。
In this study,the effect of heat treatment at different temperatures(60–100℃)on the stability of liver ferritin from Acipenser baerii(ABLF)was investigated by electrophoresis,ultraviolet(UV)absorption spectroscopy,dynamic light scattering(DLS),Fourier transform infrared(FTIR)spectroscopy.Besides,the effect of heat treatment temperatures of 25–65℃on the rate of iron release from ABLF and the storage stability of ABLF at 4 and 25℃were explored.The results showed that the solubility and iron content of ABLF gradually decreased,and the content of protein aggregates increased with increasing heat treatment temperature(60–100℃).The FTIR spectroscopic results showed that the relative content ofα-helix structure gradually decreased,and the relative content of random coil structure generally increased with increasing heat treatment temperature,especially in the range of 80–100℃,while little changes were observed in the range of 60–80℃indicating that ABLF had good stability at 60–80℃.The rate of iron release from ABLF increased with increasing heat treatment temperature(25–65℃).ABLF was more stable when stored at 4℃than at 25℃.The results of this research will provide a theoretical basis for the processing and utilization of ABLF.
作者
丁炳文
马贵红
李筱
朱佳倩
李蝶
冯颖琳
张志清
李树红
马翼
李美良
DING Bingwen;MA Guihong;LI Xiao;ZHU Jiaqian;LI Die;FENG Yinglin;ZHANG Zhiqing;LI Shuhong;MA Yi;LI Meiliang(College of Food Science,Sichuan Agricultural University,Ya’an 625000,China;Sichuan Runzhao Fishery Co.Ltd.,Ya’an 625000,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第8期66-73,共8页
Food Science
基金
四川省科技厅重点研发项目(20ZDYF1893)
四川省大学生创新创业训练计划项目(1921996121)
四川农业大学科研兴趣项目(2021996689
1911113425)。
关键词
鲟鱼肝脏铁蛋白
理化性质
热处理
稳定性
还原释放
liver ferritin from Acipenser baerii
physical and chemical properties
heat treatment
stability
reduction and release
