摘要
目的制备一种基于普鲁士蓝纳米花/电化学还原石墨烯复合纳米材料(PB/ErGO)修饰的电化学传感器(玻碳电极,GCE),用于人血清样品中多巴胺浓度的检测。方法采用扫描电镜、X-射线粉末衍射、循环伏安法、电化学阻抗谱对修饰材料的表面形貌、微观结构和电化学性能进行表征。系统优化富集时间、富集电位、底液pH等电化学检测条件。采用差分脉冲伏安法(DPV)对人血清中多巴胺的浓度进行定量检测。结果扫描电镜和X-射线粉末衍射证实成功制备了PB/ErGO复合纳米材料。PB/ErGO显著增大了电极的电化学活性面积,降低了电荷传递电阻。PB/ErGO/GCE的阳极峰电流约为裸电极的53倍,表明PB/ErGO对多巴胺氧化表现出协同增敏效应。最佳检测条件为富集时间240 s,富集电位0 V,pH 7.0。PB/ErGO/GCE动态响应范围为0.5~50μmol·L^(-1),检出限为0.06μmol·L^(-1),且成功实现了人血清样品中多巴胺浓度的检测。结论PB/ErGO/GCE具有低成本,高灵敏度,优异的重复性、再现性和选择性等优点,在生物样本中痕量多巴胺检测上具有实际应用前景。
Objective To develop an electrochemical sensor(glassy carbon electrode,GCE)based on Prussian blue nanoflower/electrochemically reduced graphene composite nanomaterials(PB/ErGO)to detect dopamine concentration in human serum samples.Methods The surface morphology,microstructure and electrochemical properties of the modified materials were characterized by scanning electron microscopy,X-ray powder diffraction,cyclic voltammetry and electrochemical impedance spectroscopy.The electrochemical detection parameters including accumulation time,accumulation potential and pH were optimized systematically.Differential pulse voltammetry(DPV)technique was used to quantitatively analyzed dopamine concentration in human serum.Results Scanning electron microscopy images and X-ray powder diffraction confirmed the successful preparation of PB/ErGO nanocomposites.PB/ErGO significantly increased the electrochemically active area and reduced the charge transferred resistance.The anodic peak current of dopamine at the PB/ErGO/GCE was about 53 times than that of the bare electrodes,demonstrating a synergistic enhancement effect of PB/ErGO on dopamine oxidation.The optimal accumulation time was 240 s,accumulation potential was 0 V,and pH 7.0.Moreover,PB/ErGO/GCE displayed a wide dynamic response range of 0.5~50μmol·L^(-1)with a low limit of detection of 0.06μmol·L^(-1),which was successfully used to detect the dopamine concentration in human serum samples.Conclusion PB/ErGO/GCE has obvious advantages of low cost,high sensitivity,excellent repeatability,reproducibility as well as selectivity,and application prospect in trace detection of dopamine in biological samples.
作者
夏用恢
李广利
胡长平
XIA Yong-hui;LI Guang-li;HU Chang-ping(Department of Pharmacology,School of Pharmaceutical Sciences,Central South University,Changsha 410078;Zhuzhou Institute for Food and Drug Control,Zhuzhou Hunan 412011;College of Life Sciences and Chemistry,Hunan University of Technology,Zhuzhou Hunan 412007)
出处
《中南药学》
CAS
2022年第4期779-784,共6页
Central South Pharmacy
