摘要
采用滴涂法研制了一种新型的、灵敏的测定氯霉素(CAP)的复合修饰玻碳电极(GCE)——石墨烯(GR)/碳纳米管(CNTs)修饰电极(GR/CNTs/GCE)。采用循环伏安法(CV)和方波溶出伏安法(SWSV)研究了CAP的电化学行为。结果表明,在0.1~10μmol/L和10~5000μmol/L范围内其氧化峰电流(I pa)与浓度(c:μmol/L)间具有良好的线性关系,回归方程分别为I pa=12.01 c+25.47和I pa=0.1796 c+169.1451,相关系数(R)分别为0.9978和0.9976,方法检出限(S/N=3)为0.05μmol/L。此法用于实际药物中CAP含量的快速检测,加标回收率为98.7%~101.3%,结果较满意。
A new and sensitive composite modified glassy carbon electrode(GR/CNTs/GCE)for the determination of chloramphenicol(CAP)was developed by dropping carbon nanotubes(CNTs)and graphene(GR).Electrochemical behavior of CAP was studied by cyclic voltammetry(CV)and square wave stripping voltammetry(SWSV).The results showed that a good linear relationship between oxidation peak current(Ipa:μA)of CAP and its concentration(c:μmol/L)was obtained in the range of 0.1~10μmol/L and 10~5000μmol/L,the regression equation was Ipa=12.01c+25.47 and Ipa=0.1796c+169.1451,the correlation coefficients(R)were 0.9978 and 0.9976,with the detection limit of 0.05μmol/L(S/N=3).The method was applied to the quick determination of CAP in acetaminophendrugs.Standard addition rate of return was 98.7%~101.3%and the results were satisfactory.
作者
匡云飞
李薇
杨颖群
冯泳兰
谷标
KUANG Yun-fei;LI Wei;YANG Ying-qun;FENG Yong-lan;GU Biao(College of Chemistry and Materials Science,Hengyang Normal University,Hengyong Hunan 421008,China)
出处
《衡阳师范学院学报》
2019年第6期52-56,共5页
Journal of Hengyang Normal University
关键词
方波溶出伏安法
石墨烯
玻碳电极
碳纳米管
氯霉素
square wave stripping voltammetry
graphene
glassy carbon electrode
carbon nanotubes
chloramphenicol
