摘要
A reliable and sensitive strategy which can assess nucleic acid levels in living cells would be essential for fundamental research of biomedical applications. Some nanomaterial-based fluorescence biosensors recently developed for detecting nucleic acids, however, are often with expensive, complicated and timeconsuming preparation process. Here, by using a facile bottom-up synthesis method, a two-dimensional(2 D) coordination polymer(CP) nanosheet, [Cu(tz)](Htz = 1,2,4-triazole), was successfully prepared after optimizing reaction conditions. These ultrathin CP nanosheets with thickness of 4.7 ± 1.1 nm could readily form nanosensors by assembly with DNA probes, which exhibited a low limit of detection(LOD)for p53 DNA fragment as 144 pmol/L. Furthermore, by integrating [Cu(tz)] nanosheets with hybridization chain reaction(HCR) probes, mi R-21, one kind of micro RNA upregulated in many cancer cells, can be sensitively detected with a LOD of 100 pmol/L and monitored in living cells, giving consistent results with those obtained by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) analysis.Thus [Cu(tz)] nanosheets, which not only possess much better nucleic acids sensing performance than bulk cystals, but also exhibit nucleic acid delivery functions, could be used as a novel nanoplatform in biomedical imaging and sensing applications.
基金
supported by the National Key Research and Development Program of China (No.2018YFA0902801)
the National Natural Science Foundations of China (Nos.21775169,21801259 and 21974153)
the Scientific Technology Project of Shenzhen City (No.JCYJ20200109142410170)
the Scientific Technology Project of Guangzhou City (No.202103000003)
the Guangdong Natural Science Foundation (Nos.2018A030313290,2019A1515010587)
the Guangdong Science and Technology Plan Project (No.2020B1212060077)
the Fundamental Research Funds for the Central Universities,SYSU (No.19lgpy142)。
