摘要
目的:观察养阴祛湿化瘀方对高尿酸性肾病大鼠肾功能及肾脏病理形态学的影响。方法:将54只SD雄性大鼠随机选取11只为空白组,其余大鼠采用氧嗪酸钾联合腺嘌呤灌胃法建立高尿酸性肾病大鼠模型。造模成功后,将造模成功的40只大鼠按照随机数字表法分为模型组、养阴祛湿化瘀方高剂量组、养阴祛湿化瘀方中剂量组、养阴祛湿化瘀方低剂量组及别嘌醇组,每组各8只。养阴祛湿化瘀方高剂量组、养阴祛湿化瘀方中剂量组、养阴祛湿化瘀方低剂量组分别予以养阴祛湿化瘀方37.2 g·kg^(-1)·d^(-1),18.6 g·kg^(-1)·d^(-1),9.3 g·kg^(-1)·d^(-1)灌胃,别嘌醇组予以别嘌醇片9.3 mg·kg^(-1)·d^(-1)灌胃,空白组及模型组予以等量生理盐水灌胃,每天1次,每次2 mL。测定各组大鼠肾功能[尿酸(uric acid,UA)、血尿素氮(blood urea nitrogen,BUN)、肌酐(creatinine,Cr)]水平,采用HE染色观察各组大鼠的肾脏病理改变。结果:模型组大鼠UA、Cr、BUN水平高于空白组,差异具有统计学意义(P<0.05)。各给药组UA水平低于模型组,养阴祛湿化瘀方高剂量组、养阴祛湿化瘀方中剂量组及别嘌醇组Cr水平低于模型组,养阴祛湿化瘀方中剂量组、养阴祛湿化瘀方低剂量组及别嘌醇组BUN水平低于模型组,差异均有统计学意义(P<0.05)。别嘌醇组UA水平低于中药各剂量组,养阴祛湿化瘀方中剂量组UA水平低于养阴祛湿化瘀方高剂量组、养阴祛湿化瘀方低剂量组,养阴祛湿化瘀方中剂量组Cr水平低于养阴祛湿化瘀方高剂量组、养阴祛湿化瘀方低剂量组,养阴祛湿化瘀方中剂量组BUN水平低于养阴祛湿化瘀方高剂量组,差异均有统计学意义(P<0.05)。模型组较空白组大鼠肾脏外观及肾组织病变明显,养阴祛湿化瘀方高剂量组、养阴祛湿化瘀方中剂量组及别嘌醇组肾脏外观及病理损伤均有不同程度减轻,但养阴祛湿化瘀方低剂量组改善不明显。
Objective:To observe the influence of Formula for Nourishing Yin and Removing Dampness and Resolving Blood Stasis(FNYRDRBS) on renal function and renal pathomorphology in rats with hyperuricemic nephropathy.Methods:A total of 54 male SD rats were randomly selected as the blank group,and the other rats were gavaged with potassium oxazinate combined with adenine to establish the rat model of hyperuric acid nephropathy.After successful modeling,the model rats were randomly divided into the model group,the high-dose group of FNYRDRBS,the medium dose group of FNYRDRBS,the low-dose group of FNYRDRBS,and the allopurinol group,with 8 rats in each group.The high-dose group of FNYRDRBS,the medium dose group of FNYRDRBS,the low-dose group of FNYRDRBS were given 37.2 g·kg^(-1)·d^(-1),18.6 g·kg^(-1)·d^(-1) and 9.3 g·kg^(-1)·d^(-1) FNYRDRBS by gavage respectively;and the allopurinol group was given allopurinol tablet 9.3 mg·kg^(-1)·d^(-1) by gavage.The blank group and the model group were given the same amount of normal saline by gavage,once a day,2 mL each time.The levels of renal function [including uric acid(UA),blood urea nitrogen(BUN) and creatinine(SCR)] were measured.The renal pathological changes of rats in each group were observed by HE staining.Results:The levels of UA,Cr and BUN in the model group were significantly higher than those in the blank group,and all the differences were statistically significant(P<0.05).The UA level in each administration group was lower than that in the model group;the Cr level in the high-dose group of FNYRDRBS,the medium dose group of FNYRDRBS and the allopurinol group were lower than that in the model group,and the BUN level in the medium dose group of FNYRDRBS,the low-dose group of FNYRDRBS and the allopurinol group were lower than that in the model group,and all the differences were statistically significant(P<0.05).The UA level of the allopurinol group was lower than that of each dose group of Chinese medicinals,the UA level of the medium dose group of FNYRDRBS was lower th
作者
胡苗青
周珂
梁恩军
欧阳美萍
HU Miao-qing;ZHOU Ke;LIANG En-jun;OUYANG Mei-ping(The Second Hospital of Hunan University of Chinese Medicine,Changsha,Hunan,China,410005;Hunan University of Chinese Medicine,Changsha,Hunan,China,410208)
出处
《河南中医》
2022年第5期702-706,共5页
Henan Traditional Chinese Medicine
基金
湖南省卫生和计划生育委员会科研计划课题项目(20180930)
湖南省教育厅科学研究项目(17C1222)。
关键词
高尿酸性肾病
养阴祛湿化瘀方
别嘌醇
肾功能
大鼠
hyperuricemic nephropathy
Formula for Nourishing Yin and Removing Dampness and Resolving Blood Stasis
allopurinol
renal function
rats
