摘要
目的:糖尿病是血糖水平慢性增高的一种代谢性疾病,现有研究认为炎症和氧化应激与Ⅱ型糖尿病的发病过程相关。炎症、氧化应激二者密切相连,相互促进,在体内介导了多种慢性病的发生,是诱发胰岛素抵抗,导致胰岛素相对缺乏的重要原因。近年来研究发现,维生素D与有氧运动在改善机体炎症反应、清除氧自由基等方面均有一定的作用,探究维生素D与有氧运动在炎症反应与氧化应激中的作用,不仅有助于解析糖尿病的产生原因,也将为糖尿病的预防和控制提供理论依据。本研究通过建立db/db小鼠糖尿病的有氧运动联合维生素D干预模型,拟从炎症信号通路、氧化应激、胰岛素抵抗等角度探讨其作用机制,为糖尿病的预防控制提供新的思路和实验依据。方法:将32只7周龄雄性db/db小鼠随机分为4组,即对照组(CG)、运动干预组(EG)、维生素D干预组(VG)和运动联合维生素D干预组(EVG),每组8只。将VD溶于注射级大豆油中,制成维生素D注射液,VG组与VEG组按7μg/kg剂量给予腹腔注射,CG组与VEG组给予等量大豆油注射,每周3次;EG组与VEG组进行持续八周每周5次的中等强度有氧运动干预。8周实验结束后,小鼠经禁食12小时后麻醉处死。采用ELISA试剂盒测定肝脏TNF-α、IL-6、CRP的表达。采用免疫组化和Western Blot方法检测NF-κB在肝脏中的表达。试剂盒检测肝脏中SOD、GSH-Px、MDA水平。结果:(1)在干预实验期间,相比CG组,VG组、EG组和VEG组实验小鼠摄入食物在第8周时均出现显著下降(P<0.01),第八周时VG组和VEG组饮水出现显著下降(P<0.01);8周干预结束后,与CG组相比,各干预组体重均呈现显著下降(P<0.01)。CG组空腹血糖变化相对平稳。由第4周开始,干预组的血糖开始出现降低趋势,较CG组VEG组显著下降(P<0.01);至第8周结束时,相比CG组,其他三组均有显著差异(P<0.01、P<0.01、P<0.01),VEG组下降较为明显。(2)HE�
Objective Diabetes is a metabolic disease with chronically elevated blood sugar levels.Current studies suggest that inflammation and oxidative stress are associated with the pathogenesis of typeⅡdiabetes.Inflammation and oxidative stress are closely linked and promote each other.They mediate the occurrence of a variety of chronic diseases in the body.They are an important reason to induce insulin resistance and lead to the relative lack of insulin.In recent years,studies have found that vitamin D and aerobic exercise play a certain role in improving the body’s inflammatory response and scavenging oxygen free radicals.Exploring the role of vitamin D and aerobic exercise in inflammatory reaction and oxidative stress not only helps to analyze the causes of diabetes,but also provides a theoretical basis for the prevention and control of diabetes.The aim of this study is to establish a model of aerobic exercise combined with vitamin D intervention for diabetes in db/db mice,and to explore its mechanism from inflammatory signaling pathway,oxidative stress and insulin resistance,so as to provide new ideas and experimental evidence for the prevention and control of diabetes.Methods Thirty two 7-week-old male d B/db mice were randomly divided into 4 groups:control group(CG),exercise intervention group(eg),vitamin D intervention group(VG)and exercise combined with vitamin D intervention group(EVG),with 8 mice in each group.VD was dissolved in injection grade soybean oil to make vitamin D injection.VG group and veg group were divided into 7 groupsμG/kg was given intraperitoneal injection,CG group and veg group were given the same amount of soybean oil injection,three times a week;Eg group and veg group were intervened with moderate intensity aerobic exercise 5 times a week for 8 weeks.At the end of the 8-week experiment,the mice were killed under anesthesia after fasting for 12 hours.Liver TNF was determined by ELISA kit-α、Expression of IL-6 and CRP.NF was detected by immunohistochemistry and Western blot-κExpressi
作者
石新宇
Shi Xinyu(Hunan University of Science and Technology,Xiangtan 411201,Hunan,China)
出处
《体育科技文献通报》
2022年第3期262-268,共7页
Bulletin of Sport Science & Technology
关键词
维生素D
运动
Ⅱ型糖尿病
胰岛素抵抗
炎症
氧化应激
vitamin D
exercise
typeⅡdiabetes
insulin resistance
inflammation
oxidative stress
