摘要
以孔石莼多糖(Ulva pertusa polysaccharides,UP)为参照,探讨孔石莼多糖锌(Ulva pertusa polysaccharides-zinc complex,UPZ)对糖尿病小鼠糖脂代谢及体内抗氧化的干预作用。采用链脲霉素(Streptozocin,STZ)诱导建立Ⅰ型糖尿病小鼠模型,试验小鼠分为10组,包括正常组,模型组,UP及UPZ低、中、高剂量组(多糖均依次为75、150、300 mg/kg BW/d,硫酸锌为无机锌组的0.5、1、2倍)无机锌(硫酸锌)组(锌含量15 mg/kg BW/d),阳性对照组(盐酸二甲双胍300 mg/kg BW/d),持续灌胃4周,并对病鼠的血糖、血脂及肝脏抗氧化指标进行测定。结果显示,UP与UPZ可以有效改善糖尿病小鼠的糖脂代谢与体内抗氧化能力,且存在明显的量效关系。与模型组相比,UP及UPZ均能抑制病鼠的体重减轻,缓解其肝、肾脏因高血糖导致的肿大;两试样均能显著或极显著(P<0.05或P<0.01)降低病鼠空腹血糖,提高其糖耐量,改善病鼠血清中的胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)浓度等血脂指标;同时,UP与UPZ还可增强病鼠肝脏中总超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)的活性,降低丙二醛(MDA)的含量。其中,高剂量UPZ对病鼠脏器损伤的修复能力和糖脂代谢的调节能力已与阳性对照无显著(P>0.05)差异;UPZ的干预效果强于同剂量的UP,且强于锌含量相同的硫酸锌。
Taking Ulva pertusa polysaccharides(UP)as control,this paper studied the intervention effects of Ulva pertusa polysaccharides-zinc complex(UPZ)on glucose-lipid metabolism and antioxidant capacity of diabetic mice.The typeⅠdiabetic model of mice was established by injecting streptozotocin(STZ).The experiment mice were divided randomly into 10 groups,including normal control,diabetic model,UP and UPZ with low,middle and high dose(polysaccharide:75,150,300 mg/kg BW/d,zinc sulfate:0.5,1,2 times of inorganic zinc group respectively),inorganic zinc(zinc sulfate,15 mg/kg BW/d Zn2+),metformin hydrochloride(300 mg/kg BW/d).All mice were subjected to intragastic administration for4 weeks.Then the blood glucose,blood lipid and antioxidant capacity of liver of diabetic mice were determined.The results showed that UP and UPZ could significantly improve the glucose-lipid metabolism and antioxidant capacity of diabetic mice.The dose-effect relationship was obvious.Compared with model group,UP and UPZ could relieve the weight loss of diabetic mice,and reduce the liver and kidney indexes of diabetic mice.Both of them could significantly(P<0.05 or P<0.01)decrease fasting blood glucose,improve glucose tolerance,and lower cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)levels,increase high density lipoprotein cholesterol(HDL-C)levels in serum of diabetic mice.Moreover,two samples could both improve the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT),reduce malondialdehyde(MDA)content in liver of diabetic mice.Furthermore,there was no significant difference(P>0.05)between the effects of high dose UPZ and metformin hydrochloride on organ injuries and glucose-lipid metabolism of diabetic mice.In addition,the effect of UPZ was stronger than UP at the same dose,and also stronger than zinc sulfate with the same zinc content.
作者
汤陈鹏
吕峰
刘伊娜
TANG Chen-peng;LV Feng;LIU Yi-na(College of Food Science Fujian Agriculture and Forestry University,Fuzhou 350002,Chin)
出处
《食品工业科技》
CAS
北大核心
2020年第1期295-300,309,共7页
Science and Technology of Food Industry
基金
福建省科技重大专项(2011NZ01010064)
福建农林大学创新基金项目(CXZX2017016)
关键词
Ⅰ型糖尿病
孔石莼多糖锌
糖脂代谢
体内抗氧化
干预
typeⅠdiabetes
Ulva pertusa polysaccharides-zinc complex(UPZ)
glucose-lipid metabolism
antioxidant capacity
intervention
