摘要
目的 探究非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清微小RNA(miR)-23a,miR-29a水平表达对靶向治疗效果的预测价值。方法 选取2015年12月~2019年6月解放军庐山康复疗养中心收治的NSCLC患者102例为观察组,选择同期该院健康体检者102例为对照组。所有NSCLC患者进行靶向治疗并分析其临床疗效;采用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)法检测血清miR-23a,miR-29a表达水平;采用受试者工作特征曲线(receiver operating characteristic curves,ROC曲线)分析血清miR-23a,miR-29a水平对靶向治疗效果的预测价值。结果 观察组血清miR-23a(1.26±0.31)和miR-29a(1.35±0.42)表达水平均明显高于对照组(0.98±0.28和1.01±0.30),差异有统计学意义(t=6.770,6.653,均P <0.05)。血清miR-23a和miR-29a表达水平均与NSCLC患者临床分期、分化程度有关(t=2.128,2.127,均P <0.05)。102例NSCLC患者经治疗后,完全缓解11例,部分缓解39例,病情稳定31例,疾病进展21例,总有效(完全缓解+部分缓解)50例(49.02%),无效(病情稳定+疾病进展)52例(50.98%)。治疗后,NSCLC患者血清miR-23a(1.08±0.22)和miR-29a(1.11±0.31)表达水平均明显低于治疗前(1.26±0.31和1.35±0.42),差异均有统计学意义(t=4.782,4.643,均P <0.05),且治疗有效患者血清miR-23a(1.05±0.21)和miR-29a(1.08±0.28)表达水平均明显低于治疗无效患者(1.20±0.29和1.27±0.31),差异均有统计学意义(t=2.982,3.244,均P <0.05);血清miR-23a和miR-29a及二者联合预测靶向治疗效果的曲线下面积分别为0.688,0.768和0.821,敏感度分别为72.0%,66.0%和94.0%,特异度分别为63.5%,78.8%和55.8%。结论 NSCLC患者血清miR-23a和miR-29a表达水平均升高,但靶向治疗后二者表达水平均降低,检测二者水平有助于预测NSCLC患者靶向治疗效果。
Objective To explore the predictive value of serum microRNA(miR)-23a and miR-29a levels in patients with nonsmall cell lung cancer(NSCLC) for targeted therapy. Methods A total of 102 cases of NSCLC patients admitted to Lushan Rehabilitation and Recuperation Center of Chinese PLA from December 2015 to June 2019 were selected as the observation group, and 102 cases of healthy people in the hospital during the same period were selected as the control group. All NSCLC patients were treated with targeted therapy and the clinical effcacy was analyzed.The expression levels of miR-23a and miR-29a in serum were detected by real-time fuorescence quantitative PCR(qRT-PCR). Receiver operating characteristic curve(ROC curve) was used to analyze the predictive value of serum miR-23a and miR-29a levels for targeted therapy. Results The levels of serum miR-23a(1.26 ± 0.31) and miR-29a(1.35 ± 0.42) in the observation group were significantly higher than those in the control group(0.98 ± 0.28 and 1.01 ± 0.30), the difference was statistically significant(t = 6.770,6.653, all P < 0.05).The expression levels of miR-23a and miR-29a were correlated with clinical stage and differentiation degree of NSCLC patients(t=2.128,2.127,all P < 0.05).After treatment, there were 11 cases of complete remission, 39 cases of partial remission, 31cases of stable disease, 21 cases of disease progression, 50 cases(49.02%) of total effective(complete remission + partial remission), 52 cases of ineffective(stable disease + disease progression)(50.98%), and after treatment, the levels of serum miR-23a(1.08 ± 0.22) and miR-29a(1.11 ± 0.31) in NSCLC patients were significantly lower than those before treatment(1.26 ±0.31 and 1.35 ± 0.42), the difference was statistically significant(t = 4.782,4.643, all P < 0.05), and the levels of serum miR-23a(1.05 ± 0.21) and miR-29a(1.08 ± 0.28) in patients with effective treatment were significantly lower than those in patients with ineffective treatment(1.20 ± 0.29 and 1.27 ± 0.31), the difference was stat
作者
胡鑫
朱娜
王琴
HU Xin;ZHU Na;WANG Qin(Department of Respiratory Medicine,Lushan Rehabilitation and Recuperation Center of Chinese PLA/Former 171 Hospital of PLA,Jiangxi Jiujiang 332000,China)
出处
《现代检验医学杂志》
CAS
2022年第2期61-65,共5页
Journal of Modern Laboratory Medicine
