摘要
长芒苋是一种原产北美洲的雌雄异株杂草。在美国,一些长芒苋种群已经对草甘膦产生了抗药性。EPSPS基因拷贝数的增加是长芒苋产生抗药性的主要原因。本文建立了双重数字PCR检测长芒苋EPSPS基因拷贝数的方法,对进口美国大豆中携带的8个长芒苋样品进行检测,发现3个样品的EPSPS相对拷贝数较低,5个样品EPSPS相对拷贝数大于30,其中1个样品EPSPS相对拷贝数高达118。由此可见,进口美国大豆中携带的长芒苋发生EPSPS基因拷贝数增加的比例很高。
Amaranthus palmeri is a dioecious weed native to North America. Some populations have developed resistance to glyphosate in the United States. EPSPS gene amplification is the main reason for the resistance of A. palmeri. This paper established a kind of duplex droplet digital PCR method to detect the copy number of the EPSPS gene of A. palmeri. 8 samples of A. palmeri contained in imported U.S. soybeans were detected. Among them,3 samples had relatively low EPSPS gene copies,5 samples had more than 30 EPSPS gene copies,while one sample had the EPSPS gene copy as high as 118. The results showed that EPSPS gene amplification occurred in a high proportion of A. palmeri among the imported U.S. soybean.
作者
伏建国
李井干
吴晶
刘晓宇
许忠祥
李洋
郭静
杨晓军
强胜
Fu Jianguo;Li Jinggan;Wu Jing;Liu Xiaoyu;Xu Zhongxiang;Li Yang;Guo Jing;Yang Xiaojun;Qiang Sheng(Nanjing Customs Animal Plant and Food Inspection Center,Nanjing 210014,China;Weed Research Laboratory,Nanjing Agricultural University)
出处
《植物检疫》
2022年第2期29-33,共5页
Plant Quarantine
基金
南京海关科研项目(2019KJ13)
江苏省“333工程”科研资助项目(BRA2019320)。
