摘要
目的探讨肠道菌群及其代谢物改变是否参与大鼠心衰的发生、发展。方法采用皮下注射异丙肾上腺素的方法诱导大鼠心衰模型,饲养6周后用超声心动图评估模型是否成功,并分为2组:心衰组和对照组。高效液相色谱串联质谱法测定两组大鼠血清中氧化三甲胺(TMAO)水平;收集两组粪便样本进行细菌DNA提取,并行16SrDNA高通量测序,使用NovoMagic云平台进行肠道菌群多样性、相对丰度、差异物种及菌群功能差异分析。结果与对照组比较,心衰组血清中TMAO水平明显升高(P<0.05),两组粪便标本的测序结果显示:在门水平上,占前3位菌种的包括厚壁菌门、拟杆菌门、放线菌门;在属水平上占前3位的菌种为乳杆菌属、Allobaculum属、杜氏杆菌属;两组菌群的多样性分析显示,心衰组与对照组大鼠肠道菌群结构相似;t检验组间物种差异分析图显示心衰组明显降低的菌群前5位为:Acidobacteriota门、维鲁士菌门、硝化螺旋菌门、Methylomirabilota门、泉古菌门(P<0.05)。其他菌群的比较差异无统计学意义(P>0.05)。LEfSe分析显示心衰组与对照组间具有显著差异(LDA绝对值>4)的菌种为鼠乳杆菌、Acidobacteriota、Bacteria。结论肠道菌群变化及其代谢物TMAO水平改变参与心衰的发生、发展。
Objective To study whether intestinal bacterial microenvironment and its metabolites are involved in the occurrence and development of heart failure in rats.Methods The heart failure model of rats was induced by subcutaneous injection of isoproterenol,and the success of the model was evaluated by echocardiography after feeding for one and a half months,and then the rats were divided into two groups:heart failure(HF)group and control group.The serum TMAO level of rats was determined by high performance liquid chromatography tandem mass spectrometry.Fecal samples of the control group and HF group were collected for bacterial DNA extraction,and then conducted 16SrDNA high-throughput sequencing.The diversity,relative abundance and different species of intestinal flora were analyzed using the NovoMagic cloud platform.Results Compared with the control group,serum TMAO level in HF group significantly increased(P<0.05).Sequencing results of fecal samples in the two groups showed that at the phylum level,the top three bacteria were Firmicutes,Bacteroidetes and Actinobacteria.At the genus level,the top three species were Lactobacillus,Allobaculum and Duchella.The diversity analysis of the two groups showed that the structure of intestinal flora in HF group was similar to that in control group.The analysis of species differences with the T test showed that the top five bacteria groups with differences between the two groups were Acidobacteriota,Verrucomicrobiota,Nitrospirota,Methylomirabilota and Crenarchaeota(P<0.05).There was no significant difference in the comparison of other flora(P>0.05).LEFSE analysis showed that Lactobacillus murinus,Acidobacteriota,Bacteria had significant differences between HF group and control group(LDA absolute value>4).Conclusion The changes of intestinal flora and its metabolites TMAO levels are involved in the occurrence and development of heart failure in rats.
作者
李丽
张昕
邹琳
Li Li;Zhang Xin;Zou Lin(Dept of Cardiac Function,The First Affiliated Hospital of Baotou Medical Collage,Baotou 014010;Graduate School of Baotou Medical Collage,Baotou 014040)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第3期407-412,共6页
Acta Universitatis Medicinalis Anhui
基金
内蒙古自治区研究生科研创新项目(编号:S20191202Z)。
关键词
心衰
肠道细菌微环境
氧化三甲胺
高通量测序
heart failure
microenvironment of intestinal bacteria
trimethylamine oxide
high throughput sequencing
