摘要
目的:探讨体外受精-胚胎移植(IVF-ET)中,应用延时摄像技术(TLI)胚胎动力学参数联合形态学评估(CMA)选择第3天(D3)胚胎对临床结局的影响。方法:回顾性分析2020年3月-2021年4月在本院行IVF-ET 443个周期,其中TLI培养213周期,常规培养230周期,均使用CMA选胚,比较两组资料及结局。TLI组移植胚胎288枚,胚胎着床(n=139)与未着床(n=149)时间参数及卵裂模式进行组间资料分析,建立选胚模型。选择65个同条件周期,TLI胚胎动力学参数联合CMA进行分层建模,首选模型A组(n=53)与次选模型B组(n=12),与TLI组、常规组比较临床结局。结果:TLI组正常受精率较常规组高(P<0.05)。TLI培养胚胎动力学参数t4≤37.0h、t8≤57.0h、s2≤1.0h、s3≤5.5h及正常卵裂模式与胚胎着床率密切相关。建模后数据较TLI组及常规组正常受精率、利用胚胎率、优胚率、妊娠率、种植率有显著性提高,流产率下降;模型中A组优质囊胚率及临床妊娠率(56.0%、73.6%)高于B组(P<0.05)。结论:应用TLI动力学参数联合CMA评估,初步建立的分层优选胚胎模型,可以改善D3胚胎移植的临床结局。
Objective:To explore the influence of the selection of the 3rd day(D3)embryos of the women based on embryo kinetic parameter by time-lapse imaging(TLI)technology combined with morphological evaluation(CMA)during in vitro fertilization-embryo transfer(IVF-ET)on their pregnancy outcomes.Methods:A retrospective analysis was conducted in 443 cycles of the women during IVF-ET,which included 213 cycles with TLI technology cultured in study group and 230 cycles with routinely cultured in control group.CMA was used to select the embryos in the two groups.The data and the pregnancy outcomes of the cycles were compared between the two groups.In the study group,there were 288 embryos had been transferred,and the time parameters and cleavage mode of 139 embryos with implantation(in group 1)and of 149 embryos without implantation(in group 2)were analyzed.An embryo selection model was established.65 cycles with the same condition were selected for hierarchical modeling by TLI embryo dynamic parameters combined with CMA.53 cycles with the first model were in group A,and 12 cycles with the second model were in group B.The clinical outcomes of the women were compared between the study group and the control group.Results:The normal fertilization rate of the women in the study group was significantly higher than that of the women in the control group(P<0.05).The embryos dynamic parameters after TLI technology cultured,such as t4≤37.0h,t8≤57.0h,S2≤1.0h,and S3≤5.5h,and the normal cleavage pattern of the embryos were closely related to the implantation rate of the embryos.After modeling,the normal fertilization rate,the utilization rate of the embryos,the optimal embryo rate,the pregnancy rate,and the implantation rate of the embryos were significantly higher than those of the embryos in the study group and the control group,while the abortion rate was significantly lower.The high quality blastocyst rate and the clinical pregnancy rate of the embryos in group A were 56.0%and 73.6%,respectively,which were significantly higher t
作者
王娟
田可可
闫虹
杜威
吕玉珍
WANG Juan;TIAN Keke;YAN Hong;DU Wei;LV Yuzhen(Jiaozuo Maternal and Child Health Care Hospital, Henan Province, 454000)
出处
《中国计划生育学杂志》
2022年第1期106-111,共6页
Chinese Journal of Family Planning
基金
河南省医学科技攻关计划联合共建项目(LHGJ20191363)。
关键词
体外受精-胚胎移植
延时摄像技术
形态学评估
胚胎种植率
临床妊娠率
In vitro fertilization-embryo transfer
Embryo transplantation
Time-lapse camera technology
Morphological evaluation
Embryo implantation rate
Clinical pregnant rate
