摘要
目的分析云南省男性群体Y染色体Amelogenin基因缺失情况。方法采集6000名云南省无关男性个体血样,使用PowerPlex21、STRtyper-21G和PowerPlex Y23试剂盒进行STR复合扩增,GeneMapper ID-X 1.5软件分析基因分型数据。结果统计分析发现,云南男性AMELY缺失频率约为0.033%。进一步探究缺失原因,AMELY缺失还伴随其他Y-STR基因座缺失,缺失导致的扩增失败是由于Yp11.2上连锁片段缺失。结论AMELY基因缺失具有一定的发生频率,将干扰性别鉴定,在检验时需要对缺失情况进行分析探究。
Objective To analyze the deletion of Amelogenin gene on Y chromosome in male population in Yunnan Province.Methods Blood samples of 6000 unrelated male individuals in Yunnan Province were collected,and PowerPlex;21,STRtyper-21G and PowerPlex Y23 kits were used for STR multiplex amplification.GeneMapper ID-X 1.5 software was used to analyze the genotyping data.Results The frequency of AMELY deletion in Yunnan males was about 0.033%.To explore the reasons of deletion,AMELY deletion was accompanied by other Y-STR loci deletion.The amplification failure Caused by deletion was due to the deletion of the linkage fragment on Yp11.2.Conclusion AMELY gene deletion has a certain frequency,which will interfere with gender identification.It is necessary to analyze and explore the deletion in the test.
作者
孙丽娟
叶峻杰
杜应雄
赵洁
张云
陈婷婷
SUNLijuan;YE Junjie;DU Yingxiong;ZHAO Jie;ZHANG Yun;CHEN Tingting(Population and Family Planning Research Institute of Yunnan Province,Key Laboratory of Preconception Health in Western China of National Health Commission,Key Laboratory of Fertility Regulation and Minority Birth Health Research of Yunnan Province,Kunming,Yunnan 650021,China)
出处
《中国优生与遗传杂志》
2022年第1期68-70,共3页
Chinese Journal of Birth Health & Heredity
