摘要
目的探究27个Y-STR基因座在贵州苗族群体中的遗传多样性分布。方法采集贵州苗族418名无关健康男性个体的血液样本,采用Y filer TM Plus试剂盒对27个Y-STR基因座进行复合扩增检测,根据基因型结果计算27个Y-STR基因座的等位基因频率、基因多态性、单倍型多态性等群体遗传学参数,分析贵州苗族和其他几个不同地区的少数民族群体的遗传关系。结果在418名贵州苗族个体中,一共观察到240个等位基因,等位基因频率为0.0024~0.8923,27个Y-STR基因座的GD值为0.1950~0.9128;群体遗传学分析结果表明,贵州苗族与其他民族之间具有相对较远的遗传关系。结论该27个Y-STR基因座在贵州苗族人群中具有丰富的遗传多态性。
Objective To investigate the genetic diversity distribution of 27 Y-STR loci in Guizhou Miao ethnic population.Methods This reaserch amplified 27 Y-STR loci in 418 unrelated healthy males of Miao ethnic group using Y filer TM plus kit and calculated population genetics parameters,including allele frequency,genetic diversity,haplotype diversity and so on.This study further investigated the genetic relationship between Guizhou Miao group and several other geographically different ethnic groups.Results This study observed 240 alleles in 418 Miao group individuals with the gene frequency ranging from 0.0024 to 0.8923;GD value of 27 Y-STR loci spanned from 0.1950 to 0.9128.Population genetic analysis showed that Guizhou Miao people possessed distinct genetic relationship with other ethnic groups.Conclusions 27 Y-STR loci show rich genetic polymorphisms in Guizhou Miao group population.
作者
刘媛媛
陈静
张晗
金珊珊
刘顶浪
罗雍兰
周林
季晶焱
LIU Yuanyuan;CHEN Jing;ZHANG Han;JIN Shanshan;LIU Dinglang;LUO Yonglan;ZHOU Lin;JI Jingyan(Department of Forensic Science,Guizhou Medical University,Guiyang 550004,Guizhou,China)
出处
《贵州医科大学学报》
CAS
2022年第2期163-170,共8页
Journal of Guizhou Medical University
基金
贵州省2019年省级大学生创新创业训练计划项目(20195200888)
贵州省教育厅特色领域项目(黔教合KY字〔2021〕065)。
关键词
法医学
Y-STR
贵州苗族
遗传多态性
群体遗传结构
forensic science
Y-STR
guizhou miao population
genetic diversity
population genetic structure
