摘要
目的探讨非小细胞肺癌(NSCLC)组织中缝隙连接蛋白43(connexin43,Cx43)的表达水平及其与临床病理、转移及患者生存时间的关系。方法选取行手术治疗的NSCLC患者76例,通过免疫组化法检测患者癌组织及癌旁组织中Cx43蛋白表达水平,分析其与患者临床病理、转移的关系;随访3年预后情况,比较Cx43蛋白阳性表达组与阴性表达组患者生存时间差异。结果NSCLC癌组织Cx43蛋白高表达率(35.53%)低于癌旁组织(76.32%)(P<0.05)。性别、年龄、吸烟史、肿瘤大小、病理类型与NSCLC患者癌组织中Cx43蛋白表达水平无关(P>0.05);低分化、临床分期Ⅲ期、发生淋巴结转移患者Cx43蛋白高表达率低于中高分化、临床分期Ⅰ期或Ⅱ期、未发生淋巴结转移患者(P<0.05);Logisitic回归分析显示分化程度低、临床分期高、发生淋巴结转移是导致Cx43蛋白低表达的独立危险因素。随访3年,Cx43蛋白高表达者3年无进展生存率与总生存率分别为66.67%(18/27)、77.78%(21/27)高于Cx43蛋白低表达者的42.86%(21/49)、51.02%(25/49)(P<0.05)。结论Cx43蛋白在NSCLC组织中的表达下调,Cx43蛋白表达水平与NSCLC的发生以及组织分化程度、临床分期、淋巴结转移相关,是NSCLC患者的预后生物分子标志物,Cx43蛋白低表达可预示患者不良预后。
Objective To explore the expression level of connexin 43(Cx43)in patients with non-small cell lung cancer(NSCLC)and its relationship with clinicopathology,metastasis and survival time.Methods 76 patients with NSCLC underwent surgical treatment were enrolled.The expression level of Cx43 in cancer tissues and para-cancerous tissues was detected by immunohistochemistry,and its relationship with clinicopathology and metastasis was analyzed.According to prognosis after 3 years of follow-up,differences in survival time between Cx43 positive group and Cx43 negative group were compared.Results The high expression rate of Cx43 in NSCLC tissues was lower than that in para-cancerous tissues(35.53%vs 76.32%)(P<0.05).The gender,age,smoking history,tumor size and pathological types wer not correlated with Cx43 in NSCLC patients(P>0.05).The high expression rate of Cx43 in patients with low differentiation,clinical staging at stage III and lymph node metastasis was lower than that with moderate to high differentiation,clinical staging at stage I or II,and without lymph node metastasis(P<0.05).Logisitic regression analysis showed that low differentiation,high clinical staging and lymph node metastasis were independent risk factors of low-expression Cx43.After 3 years of follow-up,3-year progression-free survival rate and overall survival rate in patients with high expression of Cx43 were 66.67%(18/27)and 77.78%(21/27),higher than those with low expression[42.86%(21/49),51.02%(25/49)](P<0.05).Conclusion The expression of Cx43 protein is down-regulated in NSCLC tissues.The expression level of Cx43 protein is related to the occurrence of NSCLC,degree of tissue differentiation,clinical staging and lymph node metastasis,which is a biomolecular marker for prognosis in NSCLC patients.Low expression of Cx43 protein can predict poor prognosis.
作者
史册
张伟华
刘艳梅
SHI Ce;ZHANG Weihua;LIU Yanmei(Shangqiu First People's Hospital,Shangqiu,476100)
出处
《实用癌症杂志》
2022年第3期358-361,共4页
The Practical Journal of Cancer
基金
河南省医学科技公关计划联合项目(编号:LHGJ20191501)。
