摘要
目的 基于网络药理学分析川芎治疗腰椎间盘退行性病变(LIDD)的潜在作用机制。方法 通过检索TCMSP数据库、查阅相关文献筛选川芎有效成分,并查询其相对应的靶点。采用GeneCards、OMIM数据库检索LIDD的靶点,将其与川芎预测的靶点进行映射以获得川芎潜在治疗LIDD的交集靶点。采用Cytoscape v3.8.2软件及String数据库构建“中药-有效成分-疾病-靶点”网络及PPI网络。采用Metascape平台对交集靶点进行GO功能和KEGG通路富集分析。结果 筛选出川芎的10个有效成分,179个靶点;LIDD 627个靶点;获得28个交集靶点。川芎治疗LIDD主要成分为蒿本内酯、新蛇床内酯、洋川芎内酯等,关键节点为IL1B、STAT3等。GO功能富集分析显示共涉及531种生物过程、15种细胞成分、31种分子功能。KEGG通路包括TNF信号通路、炎症介导的Trp通道、HIF-1信号通路等。结论 川芎主要有效成分为蒿本内酯、新蛇床内酯、洋川芎内酯等,可能通过IL1B、STAT3等节点作用于TNF信号通路、Trp通道、HIF-1信号通路进而治疗LIDD,这为后续LIDD治疗方案的优化及相关实验设计、药物开发提供了新思路。
Objective To analyze the potential action mechanism of Chuanxiong in the treatment of lumbar intervertebral disc degeneration(LIDD) based on network pharmacology. Methods TCMSP database and related literature were used to screen the effective components of Chuanxiong and query its corresponding targets. GeneCards and OMIM databases were used to retrieve the targets of LIDD, and map them with the targets predicted by Chuanxiong to obtain the intersection targets of Chuanxiong for potential treatment of LIDD. Cytoscape v3.8.2 software and String database were used to build "traditional Chinese Medicine-effective components-disease-targets" network and PPI network. Metascape platform was used for GO function and KEGG pathway enrichment analysis of intersection targets. Results Ten effective components and 179 targets of Chuanxiong were screened;LIDD had 627 targets, and 28 intersection targets were obtained. The main components of Chuanxiong in the treatment of LIDD were ligustilide, sedanolide, senkyunolide A, etc,and the key nodes were IL1B, STAT3, etc. GO function enrichment analysis showed that 531 biological processes, 15cellular components and 31 molecular functions were involved. KEGG pathway included TNF signaling pathway,inflammatory mediator regulation of Trp channels, HIF-1 signaling pathway, etc. Conclusion The main effective components of Chuanxiong, such as ligustilide, sedanolide, senkyunolide A, etc, may act on TNF signal pathway, Trp channels and HIF-1 signaling pathway to treat LIDD through IL1B, STAT3 and other nodes. The finding can provide new ideas for the optimization of subsequent LIDD treatment scheme, relevant experimental design and drug development.
作者
汪朋
WANG Peng(Hubei University of Chinese Medicine,Wuhan 430065,China)
出处
《临床医学研究与实践》
2022年第10期5-9,共5页
Clinical Research and Practice
关键词
网络药理学
川芎
腰椎间盘退行性病变
作用机制
network pharmacology
Chuanxiong
lumbar intervertebral disc degeneration
action mechanism
