摘要
目的探讨苍附导痰汤对多囊卵巢综合征(polycystic ovary syndrome,PCOS)模型大鼠卵巢细胞线粒的调节机制。方法采用皮下注射双氢睾酮(DHEA)的方法构建PCOS大鼠模型后,使用苍附导痰汤灌胃治疗。检测中药治疗对卵巢功能及卵巢细胞线粒体活性的影响,并观察卵巢局部包膜内慢病毒包被的mTOR shRNA序列表达载体卵巢包膜内注射对中药疗效的影响。结果苍附导痰汤灌胃治疗能够显著提高PCOS模型大鼠的排卵数并降低血清雄激素水平、提高Drp-1因子的蛋白表达水平、提高颗粒细胞线粒体活性并能够对细胞内的线粒体异常聚集状态起到改善作用。mTOR因子的shRNA能够拮抗苍附导痰汤的药效作用。结论苍附导痰汤治疗PCOS模型大鼠卵巢功能异常的药效机制很可能与mTOR介导的对线粒体功能的调节有关。卵巢颗粒细胞内线粒体活性及功能的变化很可能是连接细胞代谢与内分泌功能变化的关键节点。
Objective To investigate the mechanism of Cangfu Daotan Decoction on ovarian cells′mitochondrial in PCOS model rat.Methods The PCOS rat model was established by subcutaneous injection of DHEA.The PCOS model rats were treated with The Cangfu Daotan Decoction which was used for therapy by gavage.The effect of Chinese medicine treatment on ovarian function and mitochondrial activity of ovarian cells were investigated and the lentivirus-coated mTOR shRNA sequence expression vector ovarian capsule injection on Pharmacodynamic effects of Chinese medicine were also observed.Results The Cangfu Daotan Decoction can significantly increase ovulation number,decrease serum androgen level and increase Drp-1 protein expression in PCOS model rats.Chinese medicine also could improve the mitochondrial activity and improve the abnormal aggregation of mitochondria in ovarian granulosa cells.ShRNA of mTOR could antagonize the pharmacodynamic effect of Cangfu Daotan Decoction.Conclusion The pharmacodynamic mechanism of Cangfu Dao-tan Decoction in treating abnormal ovarian function in PCOS model rats may be related to the change of mitochondrial function mediated by mTOR.The changes of mitochondrial activity and function in ovarian granulosa cells may be the key point which connected the cells′me-tabolism and endocrine function.
作者
李威
陈静
樊锐锋
匡洪影
徐邱鸿
潘紫萌
LI Wei;CHEN Jing;FAN Ruifeng(The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Heilongjiang 150040,China)
出处
《医学研究杂志》
2022年第2期56-61,46,共7页
Journal of Medical Research
基金
黑龙江省中医药科研基金资助项目(ZHY2020-126)
黑龙江省自然科学基金资助项目(LH2021H095)
黑龙江中医药大学优秀创新人才基金资助项目(2018JC05)。
