摘要
目的:研究积雪草苷对人鼻咽癌CNE-2细胞生长、凋亡及凋亡通路的影响。方法:体外培养CNE-2细胞,将不同浓度的积雪草苷作用于CNE-2细胞,MTT和CCK-8法检测积雪草苷对细胞生长的影响;流式细胞术检测对细胞凋亡的影响;Western blotting检测Vimentin、NLRP3、Bcl-2和Caspase-3表达;ELISA法检测TNF-α和IL-1β含量。结果:MTT和CCK-8结果显示,积雪草苷可浓度依赖性地抑制CNE-2细胞的生长;流式细胞仪检测结果显示,积雪草苷可浓度依赖性地促进CNE-2细胞凋亡;Western blotting结果显示,积雪草苷促进Caspase-3蛋白的表达,抑制Vimentin、NLRP3和Bcl-2的表达;ELISA法结果显示,积雪草苷可浓度依赖性地降低TNF-α和IL-1β含量。结论:积雪草苷可显著促进人鼻咽癌细胞株CNE-2细胞凋亡,其机制可能与下调Vimentin,NLRP3蛋白的表达来实现的。
Objective:To investigate the effects of asiaticoside on the growth,apoptosis,apoptosis pathway in human nasopharyngeal carcinoma cell line CNE-2.Methods:The CNE-2 cells were cultured in vitro,and treated with different concentrations of Asiaticoside.The proliferation of cells were detected using MTT and CCK-8 assay,and the apoptosis rate of cells was detected using flow cytometry.The expression levels of Vimentin,NLRP3,Bcl-2 and Caspase-3 protein were determined using Western blotting,and the serum levels of TNF-αand IL-1βwere determined using ELISA.Results:The results of MTT and CCK-8 assay showed that asiaticoside could inhibit the proliferation of CNE-2 cells ina concentration-dependent manner,the results of flow cytometry showed that asiaticoside could promote the apoptosis of CNE-2 cells in a concentration-dependent manner,the results of Western blotting showed that asiaticoside promoted the expression of Caspase-3,and inhibited the expression levels of Vimentin,NLRP3 and Bcl-2,and the results of ELISA showed that the contents of TNF-αand IL-1βcould be decreased in a concentration-dependent manner.Conclusions:Asiaticoside can significantly promote the apoptosis of human nasopharyngeal carcinoma cell line CNE-2,and the mechanism may be realized by down-regulating the expression of Vimentin/NLRP3 protein.
作者
李林
付盈盈
司应明
LI Lin;FU Ying-ying;SI Ying-ming(Xiangyang Hospital of Traditonal Chinese Medicine,Xiangyang Hubei 441000,China)
出处
《蚌埠医学院学报》
CAS
2022年第3期293-296,301,共5页
Journal of Bengbu Medical College
