摘要
目的探讨复合发酵乳改善便秘小鼠的作用及其对肠道菌群、短链脂肪酸(short chain fatty acid,SCFA)、肠动力、肠黏膜屏障的影响。方法C57BL/6JNifdc小鼠27只,随机平均分为对照组、模型组及干预组。模型组及干预组连续给予洛哌丁胺灌胃2周,从第2周开始,干预组灌胃洛哌丁胺后加灌复合发酵乳连续治疗7 d。对照组给予生理盐水灌胃。测定各组小鼠摄食量、饮水量、体重变化、粪便含水率、首粒黑便时间及小肠推进率。检测小鼠结肠五羟色胺2C受体(serotonin C receptor,5-HTR2C)、闭锁小带蛋白-1(zona occludins-1,ZO-1)、组织黏蛋白-2(mucin-2,MUC-2)mRNA的表达量;Western blot检测Raf/ERK/MAPK相关蛋白质;气相色谱法检测肠道内SCFA水平;高通量测序分析肠道菌群变化。结果与对照组相比,模型组小鼠首粒黑便排出时间显著延长(P<0.01),粪便含水率、小肠推进率、结肠5-HTR2C、ZO-1 mRNA表达显著降低(P<0.01)。与模型组相比,干预组的首粒黑便排出时间显著缩短,粪便含水率、小肠推进率显著增加(P<0.05),结肠5-HTR2C、ZO-1 mRNA表达上调(P<0.05),结肠Raf/ERK/MAPK通路磷酸化增加,肠道产SCFA菌增加且肠道内SCFA含量增加。结论复合发酵乳可能通过调节肠道菌群多样性,增加产SCFA细菌丰度,并提高SCFA含量,增强结肠中Raf/ERK/MAPK通路的磷酸化上调5-HTR2C mRNA的表达,同时增加结肠中ZO-1 mRNA的表达,从而促进肠道蠕动并增强肠黏膜屏障功能,起到改善便秘的作用。
Objective To investigate the effects of compound fermented milk on intestinal microbiota,short chain fatty acid(SCFA),intestinal motility and mucosal barrier in mice with constipation.Methods Twenty-seven C57BL/6JNifdc mice were randomly divided into three groups:control group,model group and intervention group.The model group and the intervention group were given loperamide intragastrically for two weeks.Starting from the second week,the intervention group was treated with compound fermented milk for 7 d.The control group was given normal saline.Food intake,water intake,weight change,fecal moisture content,time of first-time black stool and small intestine propulsion rate were detected.Expression of serotonin C receptor(5-HTR2C),zona occludins-1(ZO-1)and mucin-2(MUC-2)at mRNA level in colon was analyzed.Western blot was used to measure the expression of Raf/ERK/MAPK-related proteins.SCFA level in intestinal tract was detected by gas chromatography.Intestinal microbiota diversity was analyzed by high-throughput sequencing.Results Compared with the control group,the first black stool excretion time was significantly prolonged in the model group(P<0.01).Moreover,fecal moisture content,small intestine propulsion rate and the expression of 5-HTR2C and ZO-1 at mRNA level in colon were significantly decreased(P<0.01).Compared with the model group,the first black stool excretion time was significantly shortened(P<0.05);fecal moisture content,small intestine propelling rate(P<0.05),the expression of colon 5-HTR2C and ZO-1 at mRNA level(P<0.05),phosphorylation of Raf/ERK/MAPK pathway in the colon,intestinal SCFA-producing bacteria and intestinal SCFA content were increased in the intervention group.Conclusions Compound fermented milk had a therapeutic effect on constipation in a mouse model by increasing the abundance of SCFA-producing bacteria and SCFA content,enhancing the phosphorylation of the Raf/ERK/MAPK pathway to up-regulate the expression of 5-HTR2C at mRNA level in the colon,and increasing the expression of ZO-1
作者
梅璐
王昊阳
张跃潇
王效聪
孙向东
杨明华
郑鹏远
Mei Lu;Wang Haoyang;Zhang Yuexiao;Wang Xiaocong;Sun Xiangdong;Yang Minghua;Zheng Pengyuan(Department of Gastroenterology,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China;Marshall B.J.Medical Research Center of Zhengzhou University,Zhengzhou 450000,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2022年第2期110-120,共11页
Chinese Journal of Microbiology and Immunology
基金
国家重点研发计划(2020YFC2006100)
河南省重点研发与推广专项科技攻关项目(182102310179)
河南省医学科技攻关项目(2018020237,2018020228)。
关键词
便秘
肠道菌群
短链脂肪酸
肠黏膜屏障
Constipation
Gut microbiota
Short chain fatty acid
Intestinal mucosal barrier
