摘要
为筛选出耐低温青花菜品种,通过分析不同青花菜品种幼苗在自然低温胁迫下的生理响应,利用综合评价方法筛选出耐低温性较强的品种。通过测定24个青花菜品种幼苗自然低温下叶片的丙二醛(MDA)、电解质渗透率、可溶性蛋白质和叶绿素这4个生理指标含量,利用它们的耐低温系数,结合主成分分析、隶属函数分析和聚类分析对其耐低温性进行综合评价和分类。结果表明,不同品种幼苗叶片的MDA、电解质渗透率、可溶性蛋白质和叶绿素含量之间均存在显著差异,且这4个单项指标间具有一定相关性,综合评价发现,24个青花菜品种按耐低温强弱可分为4类:第一类是强耐低温品种,只有1号‘绿雄90’这个品种;第二类属于中等耐低温品种包括2号‘翠绿’、3号‘绿丰’、4号‘绿华’等12个品种;第三类是弱耐低温品种包括6号‘台绿5号’、13号‘绿剑5号’、15号‘金品5号’等6个品种;第四类是低温敏感品种,包括10号‘绿美’、14号‘中青8号’、19号‘依绿’等5个品种。这些品种将为培育耐低温青花菜新种质提供材料基础。
In order to screen out chilling-tolerant broccoli cultivars, the physiological response of broccoli different cultivars seedlings was measured, and the comprehensive evaluation method was used to screen out the cultivars with higher chilling-tolerant capability. In this study, seedlings of 24 broccoli cultivars were used as materials and4 physiological indexes(including malondialdehyde(MDA), electrolyte permeability, soluble protein content and chlorophyll) in broccoli seedling leaves under natural low temperature stress were measured. Chilling tolerance coefficient(CTC) of the 4 physiological indexes were calculated and then conducted with principal components analysis, subordinate function analysis and hierarchical cluster analysis for evaluating the chilling tolerance capability and clustering. The result indicated that there were significant differences and correlations in the 4 physiological indexes of these different cultivars. Following the comprehensive evaluation, 24 broccoli cultivars were divided into 4 categories according to the chilling tolerant capability. The first category was high chilling-tolerant which included only one cultivar(No.1 ’lüxiong90’);the second category was medium chilling-tolerant which consisted of 12 cultivars(Including No.2 ’Cuilü’, No.3 ’Lüfeng5’, No.4 ’Lühua’ and so on);the third category was lower chilling-tolerant including 6 cultivars, such as No.6 ’Tailü5’, No.13 ’Lüjian5’, No.15 ’Jinpin5’ and so on;the fourth category was chilling-sensitive including 5 cultivars, such as No.10 ’Lümei’, No.14 ’Zhongqing8’, No.19 ’Yilü’ and so on. These cultivars would lay material foundation for the genetic improvement of low temperature tolerance of broccoli.
作者
袁金超
吴莎莎
吴汉花
王婷婷
徐平
王效华
戴圣杰
黄莹
曹雪
Yuan Jinchao;Wu Shasha;Wu Hanhua;Wang Tingting;Xu Ping;Wang Xiaohua;Dai Shengjie;Huang Ying;Cao Xue(College of Agriculture and Forestry Science,Linyi University,Linyi,276005;Agricultural and Rural Bureau of Lanling County,Lanling,277700;China Communications Construction Landscape(Shandong)Co.,Ltd.,Jinan,250000)
出处
《分子植物育种》
CAS
北大核心
2022年第3期977-986,共10页
Molecular Plant Breeding
基金
国家自然科学基金项目(31801868
31902018
32001575)
山东省自然科学基金项目(ZR2018PC023
ZR2019-PC016
ZR2019PC055)
大学生创新创业训练计划项目(S2020010452080)
临沂大学博士科研启动基金项目(LYDX2017BS008)共同资助。
