摘要
法呢基焦磷酸合酶基因(farnesyl pyrophosphate synthase,FPS)是萜类代谢途径中的关键酶基因,对茶叶品质风味形成至关重要.为了解茶树CsFPS编码蛋白的理化性质及其在干旱胁迫下的表达模式和光照对茶叶萎凋过程中CsFPS表达量以及倍半萜类化合物含量的影响,以‘福云6号’茶树叶片为供试材料,克隆CsFPS的CDS序列(NCBI登录号:MK138529)、gDNA及其启动子序列,并对其基因结构特点干旱处理下CsFPS的表达模式和茶叶萎凋过程中CsFPS表达模式及倍半萜类化合物含量变化进行分析.结果显示,CsFPS的gDNA全长2924 bp,开放式阅读框(ORF)长度为924 bp,共编码307个氨基酸;gDNA基因结构分析发现,CsFPS由11个外显子和10个内含子构成;顺式作用元件分析显示:CsFPS启动子上存在大量的顺式作用元件,包括光响应元件、激素响应元件、植物生长发育相关响应元件、胁迫响应元件和其他元件.表达分析显示:随着干旱程度加重,CsFPS表达量不断上升,茶叶萎凋过程中光照条件下CsFPS表达上调.白茶萎凋中,CsFPS表达量在白茶萎凋12 h时达到最高,是0 h表达量的1.591倍.GC-MS结果显示:萎凋可使α-法尼烯含量增加,且茶叶萎凋过程中α-法尼烯含量变化总体趋势与茶叶萎凋过程中CsFPS表达变化一致.因此,茶树CsFPS可能在茶树抵御干旱胁迫中发挥重要作用,同时光照可诱导茶叶萎凋过程中CsFPS表达,且CsFPS表达上调对茶叶倍半萜类香气物质的形成和积累具有正向调控作用.
As a key enzyme gene in terpenoid metabolic pathway,farnesyl pyrophosphate synthase(FPS)gene plays an important role in the formation of tea quality and flavor.The purpose of this study was to understand the effects of physical and chemical properties of the protein encoding FPS gene,its expression pattern under drought stress,and the effects of light on the expression of CsFPS gene and the content of sesquiterpenes during tea withering.The full-length CDS sequence(GenBank accession number:MK138529),gDNA and promoter sequence of CsFPS gene were cloned from Camellia sinensis cultivar‘Fuyun 6’.Moreover,the gene structure characteristics,the expression patterns of CsFPS,under drought treatment,and the expression patterns of CsFPS and content changes of sesquiterpenoids during tea withering were investigated.The results showed that the full-length gDNA of CsFPS was 2924 bp,containing a 924 bp open reading frame(ORF),which encodes a protein of 307 amino acids.The gDNA structural analysis found that the CsFPS gene was composed of 11 exons and 10 introns.In addition,the CsFPS promoter also contained a large number of cis-acting elements,including light response elements,hormone response elements,plant growth and development related response elements,stress response elements,and other response elements.The results of relative gene expression showed that the expression of CsFPS increased with the aggravation of drought,and the expression of CsFPS was up-regulated by light during tea withering.In white tea withering,the expression level of CsFPS reached the highest at 12 h,which was 1.591 times that of at 0 h.The results of gas chromatography mass spectrometry(GC-MS)showed that the process of tea withering could increase the content ofα-farnesene,and the change trend ofα-farnesene content was consistent with the change of CsFPS expression during tea withering.This study indicated that CsFPS might play an important role in drought resistance of tea plants and that light could induce the expression of CsFPS during tea
作者
田采云
周承哲
朱晨
李小桢
傅海峰
陈兰
赖钟雄
郭玉琼
TIAN Caiyun;ZHOU Chengzhe;ZHU Chen;LI Xiaozhen;FU Haifeng;Chen Lan;LAI Zhongxiong;GUO Yuqiong(College of Horticulture,Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province,Fuzhou 350002,China;Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2022年第1期40-49,共10页
Chinese Journal of Applied and Environmental Biology
基金
福建农林大学“双一流”建设科技创新能力提升培育计划(KSYLP004)
福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A01)
6.18协同创新院茶产业技术分院专项(K1520001A)
福建省高原学科建设经费(102/71201801101)资助。
