摘要
用RT-PCR方法从除虫菊花中扩增了菊酰焦磷酸合成酶(CPPase)的全长cDNA序列.测序分析结果表明,该基因编码区为1 185 bp,编码由395个氨基酸组成的多肽.CPPase能定位于叶绿体,具有质体转运肽,还具有异戊烯基转移酶家族成员所特有的2个天冬氨酸富集基序.通过引物两端的酶切位点将CPPase基因定向克隆到植物表达载体pBI 121中.经PCR和酶切鉴定分析,获得了除虫菊CPPase基因的正、反义植物表达载体pBI 121-CPSs和pBI 121-CPSas.
A full length cDNA of CPPase was amplified from Chrysanthemum cinerariaefolium flower by RT-PCR. Sequence analysis results showed that the cDNA contained a 1 185 bp ORF encoding a protein consisting of 395 amino acids. Bioinformatic analysis showed that the predicted CPPase had a putative chloroplast location signal peptide, and two Asp-rich motifs, which existed typically in members of prenyltransferase family. By studying the function and regualation of CPPase gene, sense and antisense recombinant vectors of pBI121-CPSs and pBI121-CPSas were successfully constructed.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2008年第1期86-89,107,共5页
Journal of Henan Agricultural University
基金
国家自然科学基金项目(3471214)
重庆市科委自然基金重点项目(2007BA1005)
关键词
菊酰焦磷酸合成酶
除虫菊酯
植物表达载体
Chrysanthemyl diphosphate synthase （CPPase）
pyrethrins
plant expression vector
