摘要
依据国家标准方法(GB/T 5535.2—2008)提取橡胶籽油不皂化物并测定其含量,通过气相色谱-质谱联用仪(GC-MS)对橡胶籽油不皂化物组分进行分析;并采用DPPH·和ABTS·法评价了橡胶籽油及其不皂化物的抗氧化活性。结果表明:橡胶籽油不皂化物含量为(1.44±0.03)%,共鉴定出13种物质,主要含有γ-谷甾醇(40.24%)、豆甾醇(17.20%)、3, 4-二氢-2, 2, 5, 7, 8-五甲基-2H-1-苯并芘喃-6-酚(9.53%)、4-胆甾烯-3-酮(6.64%)、角鲨烯(6.14%)、菜油甾醇(4.37%)和D-γ-(P)-生育三烯酚(3.27%)等;橡胶籽油及其不皂化物均具有良好的抗氧化活性,清除DPPH·的IC_(50)值分别为6.979 mg/mL和3.819 mg/mL,清除ABTS·的IC_(50)值分别为16.979 mg/mL和4.356 mg/mL。
The content of unsaponifiable matter in rubber seed oil was measured according to national standard GB/T 5535.2-2008. Then the components of unsaponifiable matter in rubber seed oil were further analyzed by gas chromatography-mass spectrometry(GC-MS). The antioxidant activities of rubber seed oil and its unsaponifiable components were evaluated by DPPH· and ABTS· methods. The results showed that the unsaponifiable matter content in rubber seed oil was(1.44±0.03)%, there were 13 kinds of unsaponifiable matter in rubber seed oil, mainly including γ-sitosterol(40.24%), stigmasterol(17.20%), 3, 4-dihydro-2, 2, 5, 7, 8-pentamethyl-2 H-1-benzopyrene-6-phenol(9.53%), 4-cholesterin-3-ketone(6.64%), squalene(6.14%), campesterol(4.37%), D-γ-(P)-tocotrienol(3.27%) and etc. Rubber seed oil and its unsaponifiable matter had good antioxidant activity, and their IC_(50)values of DPPH· scavenging rate were 6.979 mg/mL and 3.819 mg/mL, their IC_(50)values of ABTS· scavenging rate were 16.979 mg/mL and 4.356 mg/mL, respectively.
作者
苏悦
杨晶晶
刘云
胡祥
杨文仪
阚欢
SU Yue;YANG Jingjing;LIU Yun;HU Xiang;YANG Wenyi;KAN Huan(College of Life Science,Southwest Forestry University,Kunming 650224,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2021年第12期118-122,共5页
China Oils and Fats
基金
云南森林培育协同创新中心(501355)。
关键词
橡胶籽油
不皂化物
组分分析
抗氧化活性
rubber seed oil
unsaponifiable matter
component analysis
antioxidant activity
