摘要
目的:研究盾叶木Macaranga adenantha枝的化学成分并进行小鼠腹腔巨噬细胞TNF-α分泌抑制活性筛选。方法:用各种硅胶和凝胶色谱分离化合物,用波谱学方法鉴定结构;用MTT法测定化合物对小鼠腹腔巨噬细胞TNF-α分泌抑制活性。结果:从盾叶木枝的乙醇提取物中分离得到10个化合物,分别鉴定为cleomiscosin A(1)、cleomiscosin B(2)、鞣花酸-4-O-α-D-吡喃鼠李糖苷(3)、鞣花酸-4-O-β-D-吡喃木糖苷(4)、香草酸(5)、(24R)-豆甾-4-烯-3-酮(6)、(24R)-豆甾-3,6-二酮(7)、(24R)-6β-羟基-豆甾-4-烯-3-酮(8)、胡萝卜苷(9)和β-谷甾醇葡萄糖苷-6′-O-十七烷酸酯(10);在10μmol.L-1浓度下,化合物1,3和4对小鼠腹腔巨噬细胞TNF-α分泌的抑制率分别为57.4%,57.0%和64.4%。结论:化合物10为首次从大戟科中分离得到,化合物1～10均为首次从该属植物中得。化合物1,3和4对小鼠腹腔巨噬细胞TNF-α分泌表现出较强的抑制作用。
To investigate the chemical constituents of the brach of Macaranga adenantha, and evaluate their TNF-a inhibitory activity. Method： The chemical conshifuents were isolated and purified by chromatographic methods. Structures of the compounds were identified by spectroscopic methods. The TNF-a secretion inhibitory activity of the mouse peritoneal macrophages was evaluated by MTT methods. Result： Ten compounds were isolated and their structures were identified as： cleomiscosin A （1）, cleomiscosin B （2）, ellagic acid 4-O-a-D-rhamnopyranside （3）, ellagic acid 4-O-β-D-xylopyranoside （4）, vanillic acid （5）, （24R）-stigmast-4-en-3-one （6）, （24R） -stigmast-3, 6-dione （7）, （24R）-6β-hydroxy-stigmast-4-en-3-one （8）, daucosterol （9）,β-sitosteryl glucoside-6＇-O-heptadecoicate （10）. At a concentration of 10 μmol · L^-1, compounds 1, 3 and 4 showed inhibitory activity to TNF-a secretion of the mouse peritoneal macrophages with the inhibitory rates of 57. 0%, 64.4% , and 57. 4%, respectively. Condusion： All compounds were isolated from genus Macaranga for the first time. Compounds 1,3, and 4 were active against TNF-a secretion of the mouse peritoneal macrophages.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第12期1175-1179,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重点项目(20432030)
教育部"新世纪优秀人才支持计划"
