摘要
通过TCS(三氯生)亚致死剂量急性暴露斑马鱼胚胎至幼鱼,探究其对斑马鱼胚胎发育和脂质代谢的影响.结果表明,在体视显微镜下统计发现TCS在200μg/L浓度急性暴露延迟了72hpf胚胎的孵化,孵化率下降13.24%,而畸形率提高80%以上.120hpf(受精后小时)幼鱼畸形表现为能量淤积、卵黄囊肿、吸收障碍而后延,游囊闭合,心包囊肿,脊椎弯曲,少数个体小眼畸形.利用Metascape在线数据库进行TCS作用靶的预测和分析,并对药靶分子GO(基因本体)功能聚类和KEGG(京都基因与基因组百科全书)代谢途径分析,多数基因在分子功能、生物过程和细胞组成上参与羧酸代谢、类固醇激素受体活性、脂质绑定、脂质分解与代谢过程、脂质稳态、脂质定位的调控等生物学功能,有3条KEGG信号通路与脂质代谢有直接或间接的关系.通过ELISA试剂盒和Thermo全波段酶标仪对幼鱼(120hpf)生化指标的检测发现,总胆固醇(T-CHO)与甘油三脂(TG)浓度显著升高.利用油红O染料进行脂质染色发现幼鱼肝脏、卵黄、血管有脂滴堆积现象,尤其集中于肝脏和卵黄中.脂质代谢和能量供应与运动行为密切相关,借助Noldus行为分析仪进行行为学测试,表明TCS暴露后斑马鱼自主运动活力抑制,对声光刺激敏感程度显著下降.上述现象均可被100μg/L的脱氧胆酸钠供给而减轻.预示TCS低剂量环境暴露通过影响肝胆循环诱导斑马鱼脂质代谢紊乱,造成能量供应障碍.
Herein, we explored in detail the effects of TCS acute exposure at sublethal dosage on zebrafish embryonic development and lipid metabolism. TCS at 200μg/L significantly delayed the 72-hpf embryonic hatching with a decreased hatching percentage of 13.24% and an increased deformity rate of >80%. In the TCS-exposure group(4-120 hpf), the main larval malformations were reflected in energy stasis、yolk cyst、dyssorption and swim sac closure, pericardial cyst, spinal curvature and so on. Using metascape online database, the target molecules of TCS, and the underlying GO(gene ontology) function clustering and KEGG(kyoto encyclopedia of genes and genomes) metabolic pathways were analyzed systemically. Most of the target genes were involved in various biological functions, such as carboxylate metabolism, steroid hormone receptor activity, lipid binding, lipid catabolic process,lipid homeostasis and regulation of lipid localization based on the roles in molecular function, biological process and cellular component. Three KEGG signaling pathways were found to be directly or indirectly concerned with lipid metabolism. The changes in biochemical indicators of 120-hpf larvae demonstrated that TCS exposure resulted in the significant increases of the total cholesterol(T-CHO) and triglycerides(TG) levels. By oil red O staining, we observed the prominent accumulation of lipid droplets in larval liver, egg yolk and blood vessels after exposure to TCS, especially in the liver and yolk. Lipid metabolism and energy supply are closely related to motor behavior. Using Noldus behavior analyzer, after TCS exposure, zebrafish voluntary motor activity was obviously inhibited, and the sensitivity to acoustic-optic stimulation was significantly decreased. The aforementioned toxic effects could be alleviated upon addition of 100μg/L sodium deoxycholate. These findings provide compelling evidence that low-dose TCS exposure induced zebrafish lipid-metabolism disorders by affecting hepatobiliary circulation and causing energy-supply obstacl
作者
王杨
吴国辉
钱秋慧
赵晨曦
闫瑾
王学东
王慧利
WANG Yang;WU Guo-hui;QIAN Qiu-hui;ZHAO Chen-xi;YAN Jin;WANG Xue-dong;WANG Hui-li(School of Environmental Science and Engineering,Suzhou University of Science andTechnology,Suzhou 215009,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2022年第3期1394-1400,共7页
China Environmental Science
基金
国家自然科学基金资助项目(31770552)。
关键词
三氯生
斑马鱼
脂质代谢
运动行为
脱氧胆酸钠
triclosan
zebrafish
lipid metabolism
movement behavior
sodium deoxycholate
