摘要
选用斑马鱼作为模式生物,以探究阿奇霉素(AZM)急性暴露下对斑马鱼幼鱼的毒性效应.结果表明,在AZM急性暴露下,斑马鱼幼鱼出现脊柱弯曲、游囊发育不完整、卵黄囊肿现象.在神经系统方面,研究发现斑马鱼脑内神经元数量明显减少、AChE活性降低和光暗节律失衡.进一步地,使用PharmMapper网站预测AZM可能靶向的基因,并对这些靶基因进行GO和KEGG富集分析,结果显示,AZM可能还具有影响斑马鱼血管和免疫系统的潜力.更进一步的实验验证表明,AZM暴露确实导致了斑马鱼幼鱼血管分支异常以及免疫系统功能紊乱的现象.
In this study,zebrafish served as a model organism to meticulously investigate the toxicological effects of azithromycin(AZM)on larval development during acute exposure.Our research delineated several distinct pathological changes in the larvae,including spinal curvature,incomplete swim bladder development,and the formation of yolk cysts.Notably,profound neurological impacts were also observed,characterized by a significant reduction in the number of neurons within the zebrafish brain,diminished AChE activity,and disruptions in light-dark rhythms.Further investigation using the PharmMapper website identified potential AZM target genes.Subsequent GO and KEGG enrichment analysis unveiled the potential influence of AZM on zebrafish vascular and immune systems.Experimental validation substantiated these predictions,demonstrating that AZM exposure induces abnormal vascular branching and dysfunction in the immune system of zebrafish larvae.
作者
蒲倩
张尚祎
陈晨
刘兴成
王慧利
钱秋慧
PU Qian;ZHANG Shang-yi;CHEN Chen;LIU Xing-cheng;WANG Hui-li;QIAN Qiu-hui(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2024年第3期1671-1678,共8页
China Environmental Science
基金
国家自然科学基金资助项目(32071617)
江苏省“双创博士”项目(JSSCBS20210723)。
关键词
阿奇霉素
神经毒性
血管毒性
免疫毒性
斑马鱼
azithromycin
neurotoxicity
vascular toxicity
immunotoxicity
zebrafish
