摘要
以‘泉淮1547’茎段为外植体材料,建立淮山离体再生体系。结果表明:顶芽及以下1~2芽位适合作为芽诱导材料,在0.1%HgCl_(2)溶液中处理10 min,污染率分别为21.2%和25.5%,分化率分别为54.7%和49.6%;在MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+GA30.5 mg·L^(-1)+蔗糖30 g·L^(-1)+琼脂6 g·L^(-1)培养基中诱导不定芽效果较好,平均芽数4.62个;MS+6-BA 0.5 mg·L^(-1)+KT 0.5 mg·L^(-1)+NAA 0.3 mg·L^(-1)+GA30.5 mg·L^(-1)+蔗糖30 g·L^(-1)+琼脂6 g·L^(-1)培养基增殖效果较好;MS+IBA 0.5 mg·L^(-1)+NAA 0.5 mg·L^(-1)+CCC 0.5 mg·L^(-1)+AC 0.5 g·L^(-1)+蔗糖30 g·L^(-1)+琼脂6 g·L^(-1)为生根、壮苗培养基,生根率98.9%。
A regeneration system was established using the stems of Dioscorea opposita‘Quanhuai 1547’as explants.The results showed that the terminal bud and the following first and second bud positions were suitable for bud induction.When treated in 0.1% HgCl_(2) solution for 10 minutes,the infection rate were 21.2% and 25.5%,the budding rate were 54.7% and 49.6%,respectively.The optimized medium for generating adventitious buds was MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+GA30.5 mg·L^(-1)+sucrose 30 g·L^(-1)+agar 6 g·L^(-1) to reach an average bud number of 4.62;that for the proliferation,MS+6-BA 0.5 mg·L^(-1)+KT 0.5 mg·L^(-1)+NAA 0.3 mg·L^(-1)+GA30.5 mg·L^(-1)+AC 0.5 g·L^(-1)+sucrose 30 g·L^(-1)+agar 6 g·L^(-1);and that for rooting,MS+IBA 0.5 mg·L^(-1)+NAA 0.5 mg·L^(-1)+CCC 0.5 mg·L^(-1)+AC 0.5 g·L^(-1)+sucrose 30 g·L^(-1)+agar 6 g·L^(-1) with 98.9% of rooting rate.
作者
黄枝英
HUANG Zhi-ying(Quanzhou Institute of Agricultural Science,Quanzhou,Fujian 362212,China)
出处
《东南园艺》
2021年第5期24-30,共7页
Southeast Horticulture
基金
福建省科技计划项目(2018N0051)。
关键词
‘泉淮1547’淮山
组织培养
正交试验
植株再生
Dioscorea opposita‘Quanhuai 1547’
Tissue culture
Orthogonal test
Plant regeneration
