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外泌体miRNA-148a-3p在胃肠间质瘤中的表达及临床意义

Expression and clinical significance of miRNA-148a-3p in exosomes in gastrointestinal stromal tumors
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摘要 目的研究胃肠间质瘤患者血清外泌体中miRNA-148a-3p的表达情况及其与患者临床资料之间的关系。方法选择2020年6月—2021年6月本院收治的37例间质瘤患者和10名体检的健康人作为研究对象,分为间质瘤患者组和正常组两组。利用超速离心法提取间质瘤患者和健康人血清中的外泌体,采用高通量测序和qRT-PCR检测外泌体中miRNA-148a-3p的表达情况,并分析其表达和临床资料之间的关系。结果间质瘤患者血清外泌体中miRNA-148a-3p的表达水平较正常人明显升高,血清外泌体miRNA-148a-3p的水平与间质瘤患者性别、年龄无明显差异(P>0.05),与肿瘤大小、核分裂象、危险度分级呈正相关(P<0.05)。结论外泌体miRNA-148a-3p在间质瘤患者中特异性高表达,并且和预后不佳相关,提示外泌体miRNA-148a-3p可能成为间质瘤诊断和判断预后的标志物。 Objective To study the expression of miRNA-148 a-3 p in serum exosomes of patients with gastrointestinal stromal tumors and its relationship with clinical data.Methods A total of 37 patients with stromal tumor those were admitted to our hospital from June 2020 to June 2021 and 10 healthy people who underwent physical examination were selected as the research objects,they were divided into normal group and stromal tumor patient group.Exosomes in serum of stromal tumor patients and healthy people were extracted by ultra-fast centrifugation method.The expression of miRNA-148 A-3 P in exosomes was detected by high-throughput sequencing and qRT-PCR,and the relationship between the expression and clinical data was analyzed.Results The expression level of miRNA-148 a-3 p in serum exosomes of patients with stromal tumor was significantly higher than that of normal controls,andthe high expression was not related to sex and age(P>0.05),but positively correlated to tumor size,mitotic figures and risk degree(P<0.05).Conclusions MiRNA-148 a-3 p in exosomes is specifically and highly expressed in patients with stromal tumors and is associated with poor prognosis,suggesting that miRNA-148 a-3 p might be used as a marker for diagnosis and prognosis of stromal tumors.
作者 姚志恒 高歌 陈名钦 欧阳海涛 魏凯凯 钱波 YAO Zhiheng;Gao Ge;Chen Mingqin;Ouyang Haitao;Wei Kaikai;Qian Bo(Department of general surgery,the second affiliated hospital of Anhui Medical University,Hefei,Anhui,230601,China.)
出处 《齐齐哈尔医学院学报》 2022年第2期101-104,共4页 Journal of Qiqihar Medical University
关键词 miRNA-148a-3p 外泌体 胃肠间质瘤 诊断 预后 miRNA-148a-3p Exosomes Gastrointestinal stromal tumor Diagnosis Prognosis
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