摘要
本研究在传统的热水浸提-乙醇沉淀法提取岩藻聚糖工艺基础上,基于岩藻糖和硫酸基团的含量及其有益生物活性的差异,对海带岩藻聚糖的提取工艺进行改进和优化,旨在获得高品质和生物活性岩藻聚糖的无乙醇的提取工艺。通过三种不同提取工艺(热水浸提-乙醇沉淀法、热水浸提-酸沉淀法、热水浸提-酸沉淀-酶解辅助法)对海带中岩藻聚糖进行提取,分别得到样品1、样品2和样品3。岩藻糖和硫酸基团的含量及生物活性(抗氧化、抑菌)分析结果表明,热水浸提-酸沉淀-酶解辅助法制备的岩藻聚糖(样品3)中岩藻糖含量(41.10%±0.87%)和硫酸基团含量(24.90%±0.15%)均显著(P<0.05)高于行业标准(SC/T 3404-2012)要求以及样品1和样品2,并且具有最佳的抗氧化和抑菌活性。利用响应面法对热水浸提-酸沉淀-酶解辅助法提取岩藻聚糖的关键工艺条件进行优化,得到最佳提取工艺参数为:浸提温度90℃,浸提时间2.0 h,液料比为40:1 mL/g,其提取得率达到9.65%±0.11%。综上所述,本研究中热水浸提-酸沉淀-酶解辅助法的岩藻聚糖提取得率及其所制备的岩藻聚糖的生物活性显著(P<0.05)高于热水浸提-乙醇沉淀法、热水浸提-酸沉淀法制备的岩藻聚糖,因此热水浸提-酸沉淀-酶解辅助法可以作为一种有效替代热水浸提-乙醇沉淀法提取岩藻聚糖的良好工艺。
In this study, based on the traditional hot water extraction-ethanol precipitation method for fucoidan, the ethanolfree extraction process of kelp fucoidan was studied in accordance with the difference in the fucose contents and sulfate groups of fucoidans and their beneficial biological activities. Three extraction methods including hot water extractionethanol precipitation method, hot water extraction-acid precipitation method, and hot water extraction-acid precipitationenzymatic hydrolysis assisted extraction method, were applied to prepare fucoidans, and three fucoidan samples(sample 1,sample 2 and sample 3) were obtained, respectively. Fucose and sulfuric acid group-contents and biological activities(antioxidant, antibacterial activities) analysis results showed that the contents of fucose and sulfate groups in fucoidan(sample 3) prepared by hot water extraction-acid precipitation-enzymatic assisted hydrolysis method were 41.10%±0.87%and 24.90%±0.15%, respectively, which were significantly higher than the Trade Standard requirements of fucoidan(SC/T3404-2012), samples 1 and sample 2. Meanwhile, the sample 3 also had the best antioxidant and antibacterial activities in three fucoidan samples. The Response surface methodology(RSM) was used to optimize the key process conditions of hot water extraction-acid precipitation-enzymatic hydrolysis assisted extraction of fucoidan. The best extraction process parameters were obtained as follows: Extraction temperature was 90℃, extraction time was 2.0 h, liquid material ratio was40:1 mL/g, and the extraction yield reached 9.65%±0.11%. In this study, raction rate of fucoidan and the biological activity of fucoidan prepared by hot water extraction-acid precipitation-enzymatic assisted hydrolysis method were significantly higher than those prepared by hot water extraction-ethanol precipitation method and hot water extraction-acid precipitation method, therefore, the hot water extraction-acid precipitation-enzymatic assisted hydrolysis method digestion would be a good
作者
陈艳红
董玉婷
啜鹏杰
陈石乔昕
姜泽东
朱艳冰
倪辉
邓尚贵
李清彪
CHEN Yanhong;DONG Yuting;CHUAI Pengjie;CHEN Shiqiaoxin;JIANG Zedong;ZHU Yanbing;NI Hui;DENG Shanggui;LI Qingbiao(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;Laboratory and Equipment Management Department,Huaqiao University,Quanzhou 362021,China;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,China;Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China;Zhejiang Xingye Group Co.,Ltd.,Zhoushan 316014,China)
出处
《食品工业科技》
CAS
北大核心
2022年第6期168-177,共10页
Science and Technology of Food Industry
基金
国家重点研发计划资助(2020YFD0900904)。
关键词
海带
岩藻聚糖
无乙醇提取工艺
抗氧化
抑菌
Laminaria japonica
fucoidan
ethanol-free extraction process
antioxidation
bacteriostasis
