摘要
采用0.15 g/L氟苯尼考(flufenicol, FFC)诱导建立肉鸡肾损伤模型,随机分成空白组、模型组、丹参多糖(SMPS)低剂量组(1.25 g/L)、SMPS中剂量组(2.50 g/L)、SMPS高剂量组(5.00 g/L)。每日记录体质量,计算平均日增体质量。于21日龄,翅下静脉采血、摘取肾脏。结果显示,FFC饮水可以降低平均日增体质量(P<0.01),升高血清中尿素氮(BUN)、肌酐(CRE)、尿酸(UA)含量(P<0.05或P<0.01)。与模型组比较,SPMS改善肾脏病理学变化,显著降低肉鸡BUN含量(P<0.05或P<0.01)、脂质过氧化物(LPO)水平(P<0.01),显著增加谷胱甘肽过氧化酶(GSH-Px)活性(P<0.05或P<0.01)。此外,SPMS显示抑制FFC诱导的NF-κB信号通路中的p65、IκKβmRNA表达水平(P<0.05或P<0.01)。5.00 g/L的SPMS可以显著提高肉鸡平均日增体质量(P<0.01),降低UA含量(P<0.05)和IL-6、IL-1β、TNF-α水平(P<0.01)。结果表明,SMPS改善FFC诱导肉鸡的药物性肾损伤机制是通过抑制氧化应激进而减少炎症因子释放。
The kidney injury model of broilers was induced by 0.15 g/L flufenicol(FFC),and the broilers were randomly divided into blank group, model group, Salvia miltiorrhiza polysaccharides low-dose group(1.25 g/L),Salvia miltiorrhiza polysaccharides medium-dose group(2.50 g/L) and Salvia miltiorrhiza polysaccharides high-dose group(5.00 g/L).The body mass was recorded daily and average daily gain was calculated.At the age of 21 days, blood was collected from the lower wing vein and the kidney was extracted.The results showed that FFC drinking water could decrease the average daily gain(P<0.01),and increase the contents of BUN,CRE,UA in serum(P<0.05 or P<0.01).Compared with model group, SPMS improved renal pathological changes, significantly decreased BUN content(P<0.05 or P<0.01),LPO level(P<0.01),and significantly increased GSH-Px activity(P<0.05 or P<0.01).In addition, SPMS inhibited the mRNA expression levels of p65 and IκKβ in FFC-induced NF-κB signaling pathway(P<0.05 or P<0.01).5.00 g/L SPMS significantly increased the average daily gain(P<0.01),decreased the UA content(P<0.05) and the levels of IL-6,IL-1β and TNF-α of broilers(P<0.01).These results show that the mechanism of SMPS improving drug-induced renal injury in broilers is to reduce the release of inflammatory cytokines by inhibiting oxidative stress.
作者
卢春雨
张璐
耿玉萌
李淑颖
史万玉
翟向和
LU Chunyu;ZHANG Lu;GENG Yumeng;LI Shuying;SHI Wanyu;ZHAI Xianghe(College of Traditional Chinese Veterinary Medicine,Hebei Agricultural University,Baoding,Hebei 071000,China;Center for Biotechnology Innovation of Hebei,Baoding,Hebei 071000,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2022年第2期337-343,共7页
Chinese Journal of Veterinary Science
基金
双一流精准畜牧学科群资助项目(1090064)。
关键词
丹参多糖
药物性肾损伤
肉鸡
Salvia miltiorrhiza polysaccharides
drug-induced renal injury
broiler
