摘要
目的探讨人胶质母细胞瘤(GBM)组织微小核糖核酸-4516(miR-4516)的表达情况及其与病人预后的关系。方法选取2015年1月~2019年5月手术切除的GBM组织89例和颅脑损伤内减压术切除的非肿瘤脑组织35例为对照组,采用qRTPCR检测miR-4516的表达水平,根据miR-4516表达水平的中位数分为高表达和低表达。GBM病人术后随访2年,记录生存情况。结果 GBM组织miR-4516表达水平[(5.32±1.75)]明显高于对照组[(1.13±0.45);P<0.01]。术后2年随访,42例(47.19%)生存,47例47(52.81%)死亡。多因素Cox回归分析显示,miR-4516高表达是GBM生存预后病例的独立危险因素(P<0.05)。生存曲线分析显示miR-4516高表达组2年累积生存率(27.45%)明显低于低表达组(73.68%;P<0.001)。结论 GBM组织miR-4516表达增高,其高表达是病人预后不良的危险因素。
Objective To investigate the expression level of microRNA-4516(miR-4516) in human glioblastoma(GBM) tissues and its relationship with the patients prognoses.Methods The expression levels of miR-4516 were detected in GBM tissues obtained from 89 GBM patients who underwent microsurgery and in non-tumor cerebral tissues obtained from 35 patients with traumatic brain injury(control group) who underwent decompression from January 2015 to may 2019,using qRT-PCR.The GBM patients were divide into high-expression and low-expression groups according the medium expression level of miR-4516.The GBM patients were followed up for 2 years and the survval status was recorded.Results The expression level of miR-4516 in GBM tissues was significantly higher than that in the control group(P<0.05).Of 89 GBM patients,42 patients(47.19%) survived and 47(52.81%) died at the end of the follow-up.Multivariate Cox regression analysis showed that high expression of miR-4516 was an independent risk factor for the poor survival of GBM patients(P<0.05).Survival curve analysis showed that the 2-year cumulative survival rate(27.45%) of the miR-4516 high expression group was significantly lower than that(73.68%) of the low expression group(P<0.001).Conclusions The expression of miR-4516 in GBM tissues is increased,and its high expression is a risk factor for poor prognosis of GBM patients.
作者
兰展
仇洪
冯磊
黄章峰
禤义
张传东
盘丽华
LAN Zhan;QIU Hong;FENG Lei;HUANG Zhang-feng;FU Yi;ZHANG Chuan-dong;PAN Li-hua(Department of Neurosurgery,Hechi Hospital Affiliated to Youjiang Medical College for Nationalities(Hechi People’s Hospital),Hechi 547000,China)
出处
《中国临床神经外科杂志》
2022年第2期107-109,共3页
Chinese Journal of Clinical Neurosurgery
