摘要
目的初步探讨miR-126抑制人胶质瘤细胞侵袭的可能机制。方法化学合成miR-126,脂质体转染人脑胶质瘤U87细胞,应用RT-PCR、Western blot检测MMP-2基因和蛋白的表达情况,并应用Transwell小室检测转染前后细胞侵袭力的变化。结果 miR-126上调后U87细胞的MMP-2基因和蛋白表达降低,并且细胞侵袭力明显降低。结论化学合成的miR-126在抑制人胶质瘤细胞侵袭过程中发挥重要作用,可能成为胶质瘤基因治疗的新靶点。
Objective To explore the mechanism underlying the inhibiting effects of miR-126 on the invasion of human brain glioma cells.Methods miR-126 was synthesized and transfected into the U87 malignant glioma cells by liposome.The relative expression of MMP-2 and MMP-9 gene was quantified by RT-PCR,the expression of MMP-2 and MMP-9 protein was detected by Western blot,and cell invasion was assessed by Transwell chamber assay.Results miR-126 inhibited the activity of MMP-2 and the invasion of human U87 glioma cells.Conclusion The chemically synthesized miR-126 plays an important role in cell invasion of human brain glioma cells,which may be a promising target for glioma gene therapy.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第3期264-266,共3页
Cancer Research on Prevention and Treatment
基金
福建省自然科学基金面上项目资助课题(2009D002)
厦门市科技局基金资助项目(3502220077055)
