摘要
以天然原料作为培养基主要成分,采用Plackett-Burman(PB)、最陡爬坡和Box-Behnken(BB)试验设计及响应面(RSM)分析,对高产纤维素酶的里氏木霉液态发酵培养基进行优化。结果表明:最优发酵培养基条件为豆饼粉添加量2.140%,麸皮添加量1.88%,蛋白胨添加量0.30%,在此条件下,里氏木霉发酵液中的纤维素酶活力可达(42.62±1.30) U/mL;发酵后期(5 d),随着里氏木霉菌丝体大量生长,纤维素酶活力不断提高;制备的纤维素粗酶液可降解玉米秸秆中的纤维素和半纤维素,产生游离的葡萄糖、木糖、阿拉伯糖、纤维二糖等可发酵性糖。
The liquid-state fermentation medium conditions of Trichoderma reesei of high yield cellulase were optimized with natural agricultural products as the main raw materials by different test design methods, such as the Plackett-Burman(PB), steepest climbing, Box-Behnken(BB) and response surface method(RSM) analysis. The results showed that the optimal fermentation medium conditions for enzyme production were soybean meal 2.140%, wheat bran 1.88%, peptone 0.30%. Under these conditions, the cellulase activity of the fermentation broth could reach(42.62±1.30) U/mL. In the later stage of fermentation(5 d), with the large growth of mycelium, the cellulase activity increased continuously. The crude enzyme solution could degrade cellulose and hemicellulose in corn straw to produce fermentable sugars such as free glucose, xylose, arabinose and cellobiose.
作者
邢胜利
宋丽丽
张志平
魏涛
马歌丽
邢进
黎园
杨旭
XING Shengli;SONG Lili;ZHANG Zhiping;WEI Tao;MA Geli;XING Jin;LI Yuan;YANG Xu(Jiangsu Hetuo Environmental Technology Co.,Ltd.,Wuxi 214000,China;College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China;Zhengzhou Key Laboratory of Metabolic Engineering and Systems Biology,Zhengzhou 450001,China)
出处
《轻工学报》
北大核心
2022年第1期20-25,共6页
Journal of Light Industry
基金
河南省科技攻关计划项目(212102110310)
河南省高等学校重点科研项目(20B416003)
郑州轻工业大学博士启动科研基金项目(0123-13501050066)。
关键词
里氏木霉
液态发酵
纤维素酶
发酵培养基
Trichoderma reesei
liquid-state fermentation
cellulase
fermentation medium
