摘要
利用苦瓜21个SSR标记和9个表型性状数据,对地理来源多样的184份苦瓜种质资源进行核心种质构建。结合基因型数据的M策略抽样法和表型数据的随机抽样法,共抽取出34份苦瓜种质资源作为核心种质。34份苦瓜核心种质保留了原始种质93.46%的等位基因和大部分地理来源。对核心种质的SSR基因型代表性进行评价,核心种质和原始种质的观测等位基因数(N_(a))、有效等位基因数(N_(e))、Shannon指数(I)、杂合性(H)、基因多样性(GD)和多态性信息含量(PIC)6个遗传多样性参数均没有显著性差异;对核心种质的表型代表性进行评价,核心种质和原始种质的均值差异百分率(MD%)为0,极差符合率(CR%)为88.30%。以上结果表明,构建的34份苦瓜核心种质在基因型和表型方面都能代表原始种质的多样性,符合核心种质的构建要求。
Taking advantage of 21 bitter gourd SSR markers and 9 phenotypic traits,this paper constructed core collection for 184 bitter gourd germplasm resources from various geographical origins;and extracted 34 accessions as core collection by combining M strategy sampling method of genotype data and random sampling method of phenotype data.These 34 bitter gourd core collection had reserved 93.46%of the alleles and most of the geographical origins of the initial germplasm.The paper also carried out evaluation on the representativeness of SSR genotype.The results showed that there were no significant differences in the 6 genetic diversity parameters between the core collection and the initial collection.They were the number of alleles(N_(a)),effective number of alleles(N_(e)),Shannon’s Information index(I),heterozygosity(H),gene diversity(GD)and polymorphism information content(PIC).The evaluation on the phenotypic representativeness of core collection showed that the mean difference percentage(MD%)was 0,and coincidence rate(CR%)was 88.30%.The above results indicated that the 34 core collection of bitter gourd could represent the diversity of initial collection in terms of genotype and phenotype,meeting the requirement of core collection construction.
作者
崔竣杰
程蛟文
曹毅
胡开林
CUI Junjie;CHENG Jiaowen;CAO Yi;HU Kailin(School of Food Science and Engineering,Foshan University,Foshan 528225,Guangdong,China;College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China)
出处
《中国蔬菜》
北大核心
2022年第2期25-32,共8页
China Vegetables
基金
广东省自然科学基金面上项目(2019A1515011939)
广东省普通高校省级基础研究和应用基础研究重点项目(2018KZDXM016)
广东省重点领域研发计划项目(2018B020202007)
广州市科技计划项目(202002020086,202102020800)
广东省科技计划项目(2019A050520002)
佛山市植物种质资源工程技术研究中心项目。
关键词
苦瓜
核心种质
SSR标记
表型性状
bitter gourd
core collection
SSR marker
phenotypic trait
