摘要
目的探讨circ-MALAT1靶向miR-101抑制膀胱癌细胞侵袭和迁移。方法将膀胱癌细胞系SW780分为si-NC组(转染si-NC)、si-circ-MALAT1组(转染si-circ-MALAT1)、miR-NC组(转染miR-NC)、miR-101组(转染miR-101 mimics)、(si-circ-MALAT1+anti-miR-NC)组(共转染si-circ-MALAT1和anti-miR-NC)、(si-circ-MALAT1+anti-miR-101)组(共转染si-circ-MALAT1和anti-miR-101)。RT-qPCR检测circ-MALAT1和miR-101表达;Transwell小室法检测细胞侵袭和迁移;Western blot检测基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)、磷酸化磷脂酰肌醇3激酶(p-PI3K)、磷酸化蛋白激酶B(p-AKT)蛋白表达;双荧光素酶报告基因实验检测circ-MALAT1和miR-101的靶向关系。结果与癌旁组织比较,膀胱癌组织中circ-MALAT1表达水平明显上调(P<0.05),miR-101表达水平明显下调(P<0.05)。与si-NC组比较,si-circ-MALAT1组中circ-MALAT1表达水平明显下调(P<0.05),侵袭、迁移细胞数明显下调(P<0.05),p-PI3K、p-AKT蛋白表达明显下调(P<0.05)。与miR-NC组比较,miR-101组中miR-101表达水平明显上调(P<0.05),侵袭、迁移细胞数明显下调(P<0.05)。与(si-circ-MALAT1+anti-miR-NC)组比较,(si-circ-MALAT1+anti-miR-101)组细胞侵袭、迁移细胞数明显上调(P<0.05),p-PI3K、p-AKT蛋白表达明显上调(P<0.05)。结论降低circ-MALAT1表达并上调miR-101抑制膀胱癌细胞系SW780的侵袭和迁移,其机制可能与PI3K/AKT信号通路有关。
Objective To explore the function of circ-MALAT1 targeting miR-101 in the inhibition of the invasion and migration of bladder cancer cells.Methods Bladder cancer cell line SW780 was divided into si-NC group(transfection si-NC),si-circ-MALAT1 group(transfection si-circ-MALAT1),miR-NC group(transfection miR-NC),miR-101 group(transfection miR-101 mimics),si-circ-MALAT1+anti-miR-NC group(co-transfection si-circ-MALAT1 and anti-miR-NC)and si-circ-MALAT1+anti-miR-101 group(co-transfection si-circ-MALAT1 and anti-miR-101).RT-qPCR was used to examine the expression of circ-MALAT1 and miR-101;Transwell method was used to detect cell invasion and migration;The matrix metalloproteinase 2(MMP-2)and matrix metalloproteinase 9(MMP-9),phosphorylated phosphatidylinositol 3-kinase(p-PI3K),phosphorylated protein kinase B(p-AKT)protein expression were measured by Western blot;Dual luciferase reporter gene experiment was applied to detect the targeting relationship between circ-MALAT1 and miR-101.Results Compared with adjacent tissues,the expression of circ-MALAT1 in bladder cancer tissue was significantly increased(P<0.05)and the expression of miR-101 was significantly decreased(P<0.05).Compared with the si-NC group,the expression of circ-MALAT1 in the si-circ-MALAT1 group was significantly reduced(P<0.05)and the counting number of invasion and migrating cells was significantly reduced(P<0.05),the expression of p-PI3K and p-AKT protein was significantly reduced(P<0.05).Compared with the miR-NC group,the expression of miR-101 in the miR-101 group was significantly increased(P<0.05)and the number of invasion as well as migrating cells were significantly reduced(P<0.05).Compared with the(si-circ-MALAT1+anti-miR-NC)group,the(si-circ-MALAT1+anti-miR-101)group showed more cell invasion and migration(P<0.05),the expression of p-PI3K and p-AKT protein was significantly increased(P<0.05).Conclusions Reduction of circ-MALAT1 expression and up-regulation miR-101 inhibit the invasion and migration of bladder cancer cells line SW780.The mechani
作者
杨芒庄
杨旭东
王晓龙
YANG Mang-zhuang;YANG Xu-dong;WANG Xiao-long(Department of Urology, Yellow River Sanmenxia Hospital,Sanmenxia 472000, China)
出处
《基础医学与临床》
2022年第3期472-478,共7页
Basic and Clinical Medicine
