摘要
目的:探讨COX-2促进结直肠癌肝转移效果及其机制,从而为结直肠癌肝转移防治提供依据。方法:建立COX-2低表达和高表达CT26结肠癌细胞株,通过注射0.2 m L的对数生长期细胞浓度为1×10~6个/mL的正常CT26结肠癌细胞悬液(A组,n=20)、COX-2高表达的CT26结肠癌细胞悬液(B组,n=20)以及COX-2低表达的结肠癌细胞悬液(C组,n=20)至脾脏制作小鼠结肠癌肝转移模型,检测比较三组小鼠移植瘤COX-2、PI3K、p-Akt表达、移植瘤体积、肝脏肿瘤大小和数量以及生存期,并分析结直肠癌肝转移小鼠COX-2、PI3K、p-Akt表达的相互关系以及三者与其移植瘤体积、肝脏肿瘤大小和数量以及生存期的关系。结果:与A组比较,B组和C组移植瘤COX-2、PI3K、p-Akt蛋白检测阳性率均升高,移植瘤体积降低,肝脏肿瘤大小和数量降低,生存期延长(P<0.05)。与B组比较,C组移植瘤COX-2、PI3K、p-Akt蛋白检测阳性率均升高,移植瘤体积降低,肝脏肿瘤大小和数量降低,生存期延长(P<0.05)。Spearman相关分析结果显示,结直肠癌肝转移小鼠移植瘤COX-2蛋白表达与其PI3K、p-Akt蛋白表达呈正相关(P<0.05),且其移植瘤COX-2、PI3K、p-Akt与其移植瘤体积、肝脏肿瘤大小和数量均呈正相关(P<0.05),与其生存期均呈负相关(P<0.05)。结论:COX-2可促结直肠癌肝转移和影响其预后,在此过程中可能涉及其活化PI3K/Akt信号通路作用。
Objective:To investigate the effect of COX-2 on the liver metastasis of colorectal cancer and its mechanism,so as to provide the basis for prevention and treatment of liver metastasis of colorectal cancer.Method:COX-2 low expression and high expression CT26 colon cancer cell line were established,and mouse colon cancer liver metastasis model was established by injecting 0.2 mL of 1×106/mL cell concentration logarithmic growth phase normal CT26 colon cancer cell suspension(group A,n=20),COX-2 high expression colorectal cancer cell suspension(group B,n=20)and COX-2 low expression colorectal cancer cell suspension(group C,n=20)into spleen.The expression of COX-2,PI3K,p-Akt,the volume of transplanted tumor,the size and number of liver tumor and the survival time of three groups were analyzed and compared.The relationship between COX-2 expression with PI3K,p-Akt expression,the volume of transplanted tumor,the size and number of liver tumors and the survival time were analyzed.Result:Compared with group A,COX-2,PI3K and p-Akt positive protein expression rate of group B and group C were increased,the volume of transplanted tumor of group B and group C were decreased,the size and number of liver tumor of group B and group C were decreased,and the survival time of group B and group C were prolonged(P<0.05).Compared with group B,COX-2,PI3K and p-Akt positive protein expression rate of group C were increased,the volume of transplanted tumor of group C were decreased,the size and number of liver tumor of group C were decreased,and the survival time of group C was prolonged(P<0.05).Spearman correlation analysis correlated with PI3K and p-Akt protein expression in colorectal cancer liver transplanted mice(P<0.05).The expression of COX-2,PI3K and p-Akt were positively correlated with the tumor volume,the size and number of liver tumors(P<0.05),and negatively correlated with the survival time(P<0.05).Conclusion:COX-2 can promote the liver metastasis of colorectal cancer and affect its prognosis.In this process,it may be involve
作者
闫康鹏
江长安
吴昆
YAN Kangpeng;JIANG Chang’an;WU Kun(Jiangxi Provincial Cancer Hospital,Nanchang 330029,China)
出处
《中国医学创新》
CAS
2017年第35期1-5,共5页
Medical Innovation of China
基金
江西省卫生计生委科技计划项目(20165427)
