摘要
目的探究精氨酸内源性合成酶精氨酸琥珀酸盐合成酶-1(argininosuccinate synthetase,ASS1)及外源性转运载体阳离子氯基酸转运蛋白-1(cationic amino acid transporter,CAT1)与结肠癌的相关性。方法通过TCGA数据库和组织芯片探究非配对和配对样本中ASS1/CAT1在癌及癌旁组织中表达及与结肠癌相关性。结果TCGA数据库分析提示非配对样本中结肠癌组织ASS1和CAT1表达均明显高于癌旁,ASS1在癌组织中的表达与TNM分期无关,CAT1的表达与TNM分期相关。组织芯片显示在配对样本中结肠癌组织及癌旁组织均有ASS1、CAT1表达。癌及癌旁组织ASS1表达无差异,CAT1则在癌组织表达明显高于癌旁组织(P=0.000);癌组织ASS1、CAT1表达均与临床病理特征无关,但癌与癌旁组织ASS1表达量比值与淋巴转移正相关(P=0.035)。结论结肠癌组织中,ASS1、CAT1均具有阳性表达,提示其同时存在精氨酸的内源性合成和外源性摄取途径。结肠癌淋巴转移和结肠癌组织及癌旁组织的ASS1表达比值正相关,但癌组织ASS1、CAT1表达与其他临床病理特征无关。结肠癌高表达CAT1,提示结肠癌精氨酸外源性摄取亢进。
Objective To explore the relationship between arginine endogenous synthetase ASS1,exogenous transporter CAT1 and colon cancer.Methods The data of expression of ASS1 and CAT1 in unpaired samples of colon cancer and paracancerous tissues were obtained from TCGA database.The expression of ASS1 and CAT1 in paired samples of colon cancer and paracancerous tissues were detected by immunohistochemical staining in tissue microarray.The correlation of ASS1 and CAT1 expression with clinicopathological features of colon cancer was analyzed.Results TCGA database analysis showed that,in unpaired samples,the expression of ASS1 and CAT1 in colon cancer tissues was significantly higher than that of paracancerous tissues.The expression of ASS1 in cancer tissues was not correlated with TNM staging,while the expression of CAT1 was correlated with TNM staging.Tissue microarray showed that,in paired samples,ASS1 and CAT1 were both expressed in colon cancer tissues and paracancerous tissues.There was no difference in the expression of ASS1 between cancer and paracancerous tissues,while the expression of CAT1 in cancer tissues was significantly higher than that in paracancerous tissues(P=0.000).The expression of ASS1 and CAT1 in cancer tissues was not correlated with clinicopathological characteristics of colon cancer,but the ratio of ASS1 expression in cancer and that in paracancerous tissues was positively correlated with lymphatic metastasis(P=0.035).Conclusion In colon cancer tissues,both ASS1 and CAT1 have positive expression,suggesting that both endogenous synthesis pathway and exogenous uptake pathway of arginine exist.High expression of CAT1 in colon cancer suggests the hyperactive exogenous uptake of arginine in colon cancer.
作者
向鹏程
屈少华
韩俊毅
XIANG Pengcheng;QU Shaohua;HAN Junyi(Department of Gastrointestinal Surgery,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai 200120,China)
出处
《同济大学学报（医学版）》
2022年第1期95-99,共5页
Journal of Tongji University(Medical Science)
基金
国家自然科学基金(82160515)
上海市浦东新区卫生和计划生育委员会重点专科项目基金(PWZzk2017-26)
上海市浦东新区科技发展基金项目(PKJ2017-Y17)。
