摘要
目的分析前期制作的实验动物病理切片质量,为提高切片制作水平提供借鉴及指导。方法随机选取1 800件切片作为研究对象,根据相关质量控制标准评分,统计坏片率并对坏片原因予以分析。结果切片中,优片率为90.17%(1 623/1 800),坏片率为9.83%(177/1 800)。其中,甲级片占比为31.50%,坏片率在可接受范围之内。结论规范取材操作,根据实验动物品种、年龄等因素,合理选择固定液和固定时间,严格控制包埋过程,提升切片技术,适时更换试剂试药等有助于改善实验动物病理切片质量,进一步提高甲级片,助力科学研究工作。
Objective The quality of pathological sections of laboratory animals made in the early stage was analyzed to provide reference and guidance for improving the level of section making. Method A total of 1 800 slices were randomly selected as the research objects,the failure rate was calculated according to the relevant quality control standards,and the causes of the failure were analyzed. Result The optimal slice rate was 90. 17%( 1 623/1 800),and the bad slice rate was 9. 83%( 177/1 800). The proportion of grade a film is 31. 50%,which should be further improved. The failure rate is within the acceptable range. Conclusion Standardized sampling operation,reasonable selection of fixed solution and fixed time according to the species and age of laboratory animals,strict control of embedding process,improvement of sectioning technology,timely replacement of reagent and other factors can help improve the quality of pathological sections of laboratory animals and facilitate scientific research.
作者
乔欣
李梦
焦昆
李胜利
QIAO Xin;LI Meng;JIAO Kun;LI Shengli(Department of Laboratory Animal Sciences,Capital Medical University,Beijing 100069,China)
出处
《实验动物科学》
2021年第6期63-65,71,共4页
Laboratory Animal Science
关键词
病理切片
质量
坏片率
制作技巧
pathological section
quality
bad slice rate
production techniques
