摘要
目的探讨经赤芝菌发酵不同时间钩吻(赤芝钩吻)的高效液相色谱(HPLC)指纹图谱与毒性的关系。方法用赤芝菌对钩吻进行双向固体发酵炮制,取发酵9、11、13、15、17、19、21、23、25、27 d(第27天取样2次)共10批次(批号S1~S10)赤芝钩吻,采用自行建立的HPLC法测定指纹图谱。将100只无特定病原体级ICR小鼠随机分为10组(每组10只,雌雄各半),以发酵11 d赤芝钩吻对小鼠的半数致死剂量为毒性测定给药浓度配制S1~S10赤芝钩吻溶液,分别给10组小鼠灌胃,观察各组小鼠的死亡情况。根据灰色关联度计算公式计算S1~S10赤芝钩吻指纹图谱共有色谱峰与毒性(对小鼠的致死率)的关联度系数,分析赤芝钩吻毒性的主要贡献成分。结果S1~S10赤芝钩吻指纹图谱共标定17个共有色谱峰;S1~S10赤芝钩吻对小鼠的致死率分别为1.00、1.00、0.80、0.70、0.60、0.60、0.50、0.40、0、0。灰色关联度分析显示,7、3、6、9、1、8、17、12号共有色谱峰与毒性的关联度系数分别为0.868、0.838、0.830、0.828、0.824、0.820、0.818和0.802,这8个色谱峰所代表的化学成分为赤芝钩吻毒性的主要贡献成分。结论赤芝钩吻随发酵时间延长毒性逐渐降低,发酵27 d时毒性最低。发酵后钩吻的毒性是多成分共同作用的结果,毒性主要贡献成分的辨识可为发酵钩吻的质量控制和发酵工艺优化提供参考。
Objective To explore the relationship between high performance liquid chromatography(HPLC)fingerprint and toxicity of Gelsemium elegans fermented by Ganoderma lucidum(Gano derma lucidum-Gelsemium elegans)for different time.Methods Gelsemium elegans was processed by biphasic solid-state fermentation with Ganoderma lucidum.A total of 10 samples of Ganoderma lucidum Gelsemium elegans were collected after fermentation for 9,11,13,15,17,19,21,23,25,27 days(sampling twice on day 27,number:S1-S10)and the fingerprints were determined by self-established HPLC.One hundred specific pathogen-free ICR mice were randomly divided into 10 groups(each group comprised 10 mice,half were male and half were female).The median lethal dose of Ganoderma lucidum-Gelsemium elegans collected after fermentation for 11 days in mice was used as the final concentration in toxicity test.S1-S10 Ganoderma lucidum-Gelsemium elegans solutions were prepared and given to 10 groups of mice respectively by gavage administration and the death of mice was observed.According to the calculation formula of grey correlation analysis,the correlation coefficients between the common peaks of S1-S10 Gano derma lucidum-Gelsemium elegans in chromatographic fingerprint and their toxicity test results(death rate in mice)was calculated and the main components contributing to the toxicity of Ganoderma lucidum-Gelsemium elegans were analyzed.Results A total of 17 common peaks were identified in the chromatographic fingerprint spectrum of S1-S10 Ganoderma lucidum-Gelsemium elegans.The mortalities in mice caused by S1-S10 of Ganoderma lucidum-Gelsemium elegans were 1.00,1.00,0.80,0.70,0.60,0.60,0.50,0.40,0,and 0,respectively.The grey correlation analysis showed that the correlation coefficients of common peak 7,3,6,9,1,8,17,and 12 to toxicity were 0.868,0.838,0.830,0.828,0.824,0.820,0.818,and 0.802,respectively.The chemical components represented by these 8 chromatographic peaks had more contribution to the toxicity of Ganoderma lucidum-Gelsemium elegans.Conclusions With
作者
黄美霞
李群
王颖峥
廖华军
王英豪
吴水生
Huang Meixia;Li Qun;Wang Yingzheng;Liao Huajun;Wang Yinghao;Wu Shuisheng(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350122,China)
出处
《药物不良反应杂志》
CSCD
2022年第1期7-12,共6页
Adverse Drug Reactions Journal
基金
福建省自然科学基金资助项目(2019J01343)。
关键词
钩吻属
发酵
动物实验
毒性试验
Gelsemium
Fermentation
Animal experimentation
Toxicity tests
